Proliferating Cell Nuclear Antigen (PCNA) antibody

Details for Product No. ABIN650229
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Antigen
Synonyms
53/13, CG9193, DmPCNA, Dmel\\CG9193, MUS209, Mus209, PCNA, PCNA/mus209, Pcna, dPCNA, l(2)02448, l(2)56F, l(2)56Fa, l(2)s1534, pcna, AO090003000846, PCNAR, Pcna/cyclin, cb16, fb36g03, wu:fa28e03, wu:fb ... show more
53/13, CG9193, DmPCNA, Dmel\\CG9193, MUS209, Mus209, PCNA, PCNA/mus209, Pcna, dPCNA, l(2)02448, l(2)56F, l(2)56Fa, l(2)s1534, pcna, AO090003000846, PCNAR, Pcna/cyclin, cb16, fb36g03, wu:fa28e03, wu:fb36g03, pcna-A show less
Reactivity
Human, Rat (Rattus)
(316), (177), (139), (78), (66), (62), (13), (13), (12), (12), (11), (8), (6), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(182), (181), (4), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(20), (17), (6), (6), (6), (6), (6), (6), (6), (6), (6), (4), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(254), (121), (78), (77), (60), (60), (53), (34), (28), (23), (4), (3), (2), (1), (1), (1)
Pubmed 2 references available
Quantity 100 µL
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Catalog No. ABIN650229
450.00 $
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Immunogen A synthetic peptide corresponding to residues in human PCNA was used as an immunogen.
Specificity A synthetic peptide corresponding to residues in human PCNA was used as an immunogen.
Alternative Name PCNA
Background Proliferating cell nuclear antigen (PCNA) is a DNA polymerase accessory factor that is required for DNA replication during S phase of the cell cycle and for resynthesis during nucleotide excision repair of damaged DNA. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway (1). The ubiquitination of PCNA is an essential event in the operation of the DNA Damage Tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase (2).
Molecular Weight 36 kDA
Gene ID 3860
UniProt P12004
Research Area Ubiquitin-related antibodies, Signaling, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:500-1000 , IHC: = 1:100-250
Comment

Background: Proliferating cell nuclear antigen (PCNA) is a DNA polymerase accessory factor that is required for DNA replication during S phase of the cell cycle and for resynthesis during nucleotide excision repair of damaged DNA. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway (1). The ubiquitination of PCNA is an essential event in the operation of the DNA Damage Tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase (2).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment PCNA Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Gary, Ludwig, Cornelius et al.: "The DNA repair endonuclease XPG binds to proliferating cell nuclear antigen (PCNA) and shares sequence elements with the PCNA-binding regions of FEN-1 and cyclin-dependent kinase inhibitor p21." in: The Journal of biological chemistry, Vol. 272, Issue 39, pp. 24522-9, 1997 (PubMed).

Zhang, Chea, Meng et al.: "PCNA is ubiquitinated by RNF8." in: Cell cycle (Georgetown, Tex.), Vol. 7, Issue 21, pp. 3399-404, 2008 (PubMed).

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