Add to Basket Bulk discount
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

Proliferating Cell Nuclear Antigen (PCNA) antibody

Antigen

Proliferating Cell Nuclear Antigen (PCNA)
Synonyms MGC8367, PCNAR, Pcna/cyclin, pcna
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN650229
Quantity 100ul  (Variants)
Price 450.00 $   Plus shipping costs $45.00
Bulk discount
Shipping to
Availability Will be delivered in 2 to 3 Business Days

Additional Information
Application Details Publications Alternatives Variants back to top

Alternative name PCNA
Gene ID 3860
UniProt P12004
Immunogen A synthetic peptide corresponding to residues in human PCNA was used as an immunogen.
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues in human PCNA was used as an immunogen.
Molecular Weight 36 kDA
Comments

Background: Proliferating cell nuclear antigen (PCNA) is a DNA polymerase accessory factor that is required for DNA replication during S phase of the cell cycle and for resynthesis during nucleotide excision repair of damaged DNA. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway (1). The ubiquitination of PCNA is an essential event in the operation of the DNA Damage Tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase (2).

Application Details
Publications Alternatives Variants back to top

Application Notes The suggested dilution is: WB: ~~ 1:500~1000 IP: ~~ 1:50 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage PCNA Antibody can be stored at -20°C for up to 12 months from time of receipt.
Research Area Ubiquitin-related antibodies, Signaling, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Gary, Ludwig, Cornelius et al.: "The DNA repair endonuclease XPG binds to proliferating cell nuclear antigen (PCNA) and shares sequence elements with the PCNA-binding regions of FEN-1 and cyclin-dependent kinase inhibitor p21." in: The Journal of biological chemistry, Vol. 272, Issue 39, pp. 24522-9, 1997 (PubMed).

Zhang, Chea, Meng et al.: "PCNA is ubiquitinated by RNF8." in: Cell cycle (Georgetown, Tex.), Vol. 7, Issue 21, pp. 3399-404, 2008 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Proliferating Cell Nuclear Antigen (PCNA)", type "Antibodies"
Hosts Rabbit (114), Mouse (104), Goat (3), Rat (2)
Reactivities Human (180), Rat (Rattus) (112), Mouse (Murine) (86), Cow (Bovine) (68), Dog (Canine) (56), Chicken (42), Pig (Porcine) (11), Sheep (Ovine) (10), Hamster (6), Guinea Pig (5), Rabbit (5), Vertebrates (5), Insect (4), Algae (3), Yeast (Saccharomyces Cerevisiae) (3), Amphibian (2), Cat (Feline) (1), Invertebrate (1), Microalgae (Isochrysis galbana) (1), Monkey (1), Vertebrate (1), Xenopus laevis (1), Yeast (Pichia pastoris) (1), Zebrafish (1)
Applications Western Blotting (WB) (127), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (73), Immunofluorescence (IF) (71), ELISA (58), Immunohistochemistry (IHC) (40), Immunoprecipitation (IP) (38), Flow Cytometry (FACS) (35), Immunohistochemistry (Fixed) (IHC (fx)) (8), Immunocytochemistry (ICC) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (2), Cellular Assay (CA) (1), Dot Blot (Dot) (1), Immunoassay (IA) (1), Immunostaining (ISt) (1)
Conjugates FITC (13), Biotin (11), Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), PE,Cy5.5 (6), PE (5), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), APC (1), RPE (1)
Epitopes C-Term (13), Internal Region (6), AA 55-71 (4), pTyr211 (4), AA 1-261 (3), Center (2), AA 111-122 (1), AA 75-125 (1), C-Term,ubLys164 (1), Internal Region,AA 111-122 (1), Nuclear (1), Tyr211 (1), pGlu1,pThr395 (1)