anti-PCNA antibody (Proliferating Cell Nuclear Antigen) (C-Term)

Details for Product anti-PCNA Antibody No. ABIN650230
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Antigen
Epitope
C-Term
(31), (24), (15), (13), (12), (12), (12), (9), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(375), (163), (119), (17), (17), (12), (11), (9), (9), (9), (9), (8), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(236), (193), (7), (2)
Clonality
Monoclonal
Conjugate
This PCNA antibody is un-conjugated
(23), (19), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(312), (136), (125), (114), (81), (72), (57), (31), (30), (26), (13), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 100 μL
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Immunogen A synthetic peptide corresponding to residues near the C terminus in human PCNA was used as an immunogen.
Specificity A synthetic peptide corresponding to residues near the C terminus in human PCNA was used as an immunogen.
Alternative Name PCNA (PCNA Antibody Abstract)
Background Proliferating cell nuclear antigen (PCNA) is a DNA polymerase accessory factor that is required for DNA replication during S phase of the cell cycle and for resynthesis during nucleotide excision repair of damaged DNA. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway (1). The ubiquitination of PCNA is an essential event in the operation of the DNA Damage Tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase (2).
Synonyms: PCNA, Proliferating cell nuclear antigen, Cyclin
Molecular Weight 36 kDA
Gene ID 7453
UniProt P12004
Research Area Ubiquitin-related antibodies, Signaling, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes IHC: = 1:50-100, WB: = 1:1000-5000
Comment

Background: Proliferating cell nuclear antigen (PCNA) is a DNA polymerase accessory factor that is required for DNA replication during S phase of the cell cycle and for resynthesis during nucleotide excision repair of damaged DNA. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway (1). The ubiquitination of PCNA is an essential event in the operation of the DNA Damage Tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase (2).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment PCNA Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Zhang, Chea, Meng et al.: "PCNA is ubiquitinated by RNF8." in: Cell cycle (Georgetown, Tex.), Vol. 7, Issue 21, pp. 3399-404, 2008 (PubMed).

Gary, Ludwig, Cornelius et al.: "The DNA repair endonuclease XPG binds to proliferating cell nuclear antigen (PCNA) and shares sequence elements with the PCNA-binding regions of FEN-1 and cyclin-dependent kinase inhibitor p21." in: The Journal of biological chemistry, Vol. 272, Issue 39, pp. 24522-9, 1997 (PubMed).

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Catalog No. ABIN650230
481.43 $
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