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Programmed Cell Death 4 (Neoplastic Transformation Inhibitor) (PDCD4) antibody

Antigen

Programmed Cell Death 4 (Neoplastic Transformation Inhibitor) (PDCD4)
Synonyms h731, MGC69337, PDCD4
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN650231
Quantity 100ul  (Variants)
Price 450.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name PDCD4
Gene ID 27250
UniProt Q53EL6
Immunogen A synthetic peptide corresponding to residues near the C-terminus of human PDCD4 was used as an immunogen.
Description Other names: PDCD4, H731, Programmed cell death protein 4, Nuclear antigen H731-like,Neoplastic transformation inhibitor protein,Protein 197/15a
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues near the C-terminus of human PDCD4 was used as an immunogen.
Molecular Weight 54 kDA
Comments

Background: Programmed cell death protein 4 (PDCD4) is a tumor suppressor that inhibits tumor promoter-induced neoplastic transformation. It down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription (1). PDCD4 inhibits the translation initiation factor eIF4A, an RNA helicase that catalyzes the unwinding of secondary structure at the 5', untranslated region (5',UTR) of messenger RNAs (mRNAs). It is also believed that regulated degradation of PDCD4 in response to mitogens allows efficient protein synthesis and consequently cell growth (2).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:500~1000 FC: ~~ 1:550 ICC: ~~ 1:250~500 IHC: ~~ 1:1000~2000
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage PDCD4 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Cancer, Signaling, Chromatin, Cell Structure
Restrictions For Research Use only

Publications
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Product Dorrello, Peschiaroli, Guardavaccaro et al.: "S6K1- and betaTRCP-mediated degradation of PDCD4 promotes protein translation and cell growth." in: Science (New York, N.Y.), Vol. 314, Issue 5798, pp. 467-71, 2006 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Programmed Cell Death 4 (Neoplastic Transformation Inhibitor) (PDCD4)", type "Antibodies"
Hosts Rabbit (59), Mouse (14), Goat (4)
Reactivities Human (79), Rat (Rattus) (42), Mouse (Murine) (41), Cow (Bovine) (6), Dog (Canine) (6), Xenopus laevis (5), Pig (Porcine) (4), Xenopus (2), Cat (Feline) (1), Chicken (1)
Applications Western Blotting (WB) (57), ELISA (45), Immunofluorescence (IF) (20), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Immunohistochemistry (IHC) (19), Immunoprecipitation (IP) (6), Flow Cytometry (FACS) (5), Fluorescence Microscopy (FM) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (3), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes pSer457 (12), C-Term (10), AA 1-469 (3), N-Term (3), AA 210-221 (2), AA 100-150 (1), AA 419-469 (1), Center (1), Internal Region (1), pSer457,Internal Region (1), pSer67 (1)