Programmed Cell Death 4 (Neoplastic Transformation Inhibitor) (PDCD4) antibody

Antigen: Programmed Cell Death 4 (Neoplastic Transformation Inhibitor) (PDCD4)

Synonyms: h731, MGC69337, PDCD4

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650232

Additional Information

Alternative name: PDCD4

Gene ID: 27250

UniProt: Q53EL6

Immunogen: A synthetic peptide corresponding to residues near the N-terminus of human PDCD4 was used as an immunogen.

Description: Other names: PDCD4, H731, Programmed cell death protein 4, Nuclear antigen H731-like,Neoplastic transformation inhibitor protein,Protein 197/15a

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the N-terminus of human PDCD4 was used as an immunogen.

Molecular Weight: 54 kDA

Comments:

Background: Programmed cell death protein 4 (PDCD4) is a tumor suppressor that inhibits tumor promoter-induced neoplastic transformation. It down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription (1). PDCD4 inhibits the translation initiation factor eIF4A, an RNA helicase that catalyzes the unwinding of secondary structure at the 5', untranslated region of messenger RNAs. It is also believed that regulated degradation of PDCD4 in response to mitogens allows efficient protein synthesis and consequently cell growth (2).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000 FC: ~~ 1:100 ICC: ~~ 1:100~250 IHC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: PDCD4 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Cancer, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Dorrello, Peschiaroli, Guardavaccaro et al.: "S6K1- and betaTRCP-mediated degradation of PDCD4 promotes protein translation and cell growth." in: Science (New York, N.Y.), Vol. 314, Issue 5798, pp. 467-71, 2006 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).