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Details for Product No. ABIN650234

PDGF beta (pTyr740) antibody

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Antigen
Epitope
»Alternatives pTyr740
Reactivity
»Alternatives Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Monoclonal
Application
»Alternatives Western Blotting (WB), Immunocytochemistry (ICC)
Pubmed 1 reference available
Catalog no. ABIN650234
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
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Immunogen A phospho specific peptide corresponding to residues surrounding Tyrosine 740 of human PDGF-Receptor β, was used as an immunogen. This antibody detects PDGF-Receptor β, phosphorylated at Tyrosine 740
Specificity A phospho specific peptide corresponding to residues surrounding Tyrosine 740 of human PDGF-Receptor β, was used as an immunogen. This antibody detects PDGF-Receptor β, phosphorylated at Tyrosine 740
Background Human platelet-derived growth factor (PDGF) receptor is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-α, and PDGF receptor-Beta. PDGF receptor-Beta can only bind two specific PDGF isoforms, PDGF-Beta and PDGF-Δ, (1,2). Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase (3), Grb2, and Src, among others. Both Tyrosine 751 and 740 is required for PDGFR-Beta mediated PI3 kinase activation. In particular, Tyrosine 751 is the docking site for P13 kinase (4).
Synonyms: PDGFRB, Beta-type platelet-derived growth factor receptor, PDGF-R-beta,CD140 antigen-like family member B
Molecular Weight 190 kDA
Gene ID 5159
UniProt P09619
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Signaling, Chromatin
Application Notes The suggested dilution is: WB: ~~ 1:1000~2000 ICC: ~~ 1:100
Comment

Background: Human platelet-derived growth factor (PDGF) receptor is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-α, and PDGF receptor-β,. PDGF receptor-β, can only bind two specific PDGF isoforms, PDGF-β, and PDGF-Δ, (1,2). Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase (3), Grb2, and Src, among others. Both Tyrosine 751 and 740 is required for PDGFR-β, mediated PI3 kinase activation. In particular, Tyrosine 751 is the docking site for P13 kinase (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment PDGF beta Antibody Phospho (pY740) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Panayotou, Bax, Gout et al.: "Interaction of the p85 subunit of PI 3-kinase and its N-terminal SH2 domain with a PDGF receptor phosphorylation site: structural features and analysis of conformational changes." in: The EMBO journal, Vol. 11, Issue 12, pp. 4261-72, 1992 (PubMed).

Alternatives for antigen "PDGF beta", type "Antibodies"
Hosts (2)
Reactivities (2)
Applications (2), (1), (1), (1)
Epitopes (1), (1)
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