PDGF beta (pTyr740) antibody

Antigen: PDGF beta

Binding Site: pTyr740

Clonality: Monoclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN650234

Additional Information

Gene ID: 5159

UniProt: P09619

Immunogen: A phospho specific peptide corresponding to residues surrounding Tyrosine 740 of human PDGF-Receptor β, was used as an immunogen. This antibody detects PDGF-Receptor β, phosphorylated at Tyrosine 740

Description: Other names: PDGFRB, Beta-type platelet-derived growth factor receptor, PDGF-R-beta,CD140 antigen-like family member B

Characteristics: Rabbit Monoclonal Antibody

Specificity: A phospho specific peptide corresponding to residues surrounding Tyrosine 740 of human PDGF-Receptor β, was used as an immunogen. This antibody detects PDGF-Receptor β, phosphorylated at Tyrosine 740

Molecular Weight: 190 kDA

Comments:

Background: Human platelet-derived growth factor (PDGF) receptor is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-α, and PDGF receptor-β,. PDGF receptor-β, can only bind two specific PDGF isoforms, PDGF-β, and PDGF-Δ, (1,2). Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase (3), Grb2, and Src, among others. Both Tyrosine 751 and 740 is required for PDGFR-β, mediated PI3 kinase activation. In particular, Tyrosine 751 is the docking site for P13 kinase (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~2000 ICC: ~~ 1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: PDGF beta Antibody Phospho (pY740) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Signaling, Chromatin

Restrictions: For Research Use only

Publications

Product: Panayotou, Bax, Gout et al.: "Interaction of the p85 subunit of PI 3-kinase and its N-terminal SH2 domain with a PDGF receptor phosphorylation site: structural features and analysis of conformational changes." in: The EMBO journal, Vol. 11, Issue 12, pp. 4261-72, 1992 (PubMed).