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Platelet Derived Growth Factor, B Polypeptide (PDGFB) (pTyr740) antibody

Details for Product No. ABIN650234
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Antigen
Epitope
pTyr740
(1), (1)
Reactivity
Rat (Rattus)
(2)
Host
Rabbit
(2)
Clonality
Monoclonal
Application
Western Blotting (WB)
(2), (1), (1)
Pubmed 1 reference available
Quantity 100 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Catalog No. ABIN650234
507.14 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A phospho specific peptide corresponding to residues surrounding Tyrosine 740 of human PDGF-Receptor β, was used as an immunogen. This antibody detects PDGF-Receptor β, phosphorylated at Tyrosine 740
Specificity A phospho specific peptide corresponding to residues surrounding Tyrosine 740 of human PDGF-Receptor β, was used as an immunogen. This antibody detects PDGF-Receptor β, phosphorylated at Tyrosine 740
Alternative Name PDGF beta
Background Human platelet-derived growth factor (PDGF) receptor is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-α, and PDGF receptor-Beta. PDGF receptor-Beta can only bind two specific PDGF isoforms, PDGF-Beta and PDGF-Δ, (1,2). Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase (3), Grb2, and Src, among others. Both Tyrosine 751 and 740 is required for PDGFR-Beta mediated PI3 kinase activation. In particular, Tyrosine 751 is the docking site for P13 kinase (4).
Synonyms: PDGFRB, Beta-type platelet-derived growth factor receptor, PDGF-R-beta,CD140 antigen-like family member B
Molecular Weight 190 kDA
Gene ID 5159
UniProt P09619
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Signaling, Chromatin
Application Notes The suggested dilution is: WB: = 1:1000-2000
Comment

Background: Human platelet-derived growth factor (PDGF) receptor is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-α, and PDGF receptor-β,. PDGF receptor-β, can only bind two specific PDGF isoforms, PDGF-β, and PDGF-Δ, (1,2). Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase (3), Grb2, and Src, among others. Both Tyrosine 751 and 740 is required for PDGFR-β, mediated PI3 kinase activation. In particular, Tyrosine 751 is the docking site for P13 kinase (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment PDGF beta Antibody Phospho (pY740) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Panayotou, Bax, Gout et al.: "Interaction of the p85 subunit of PI 3-kinase and its N-terminal SH2 domain with a PDGF receptor phosphorylation site: structural features and analysis of conformational changes." in: The EMBO journal, Vol. 11, Issue 12, pp. 4261-72, 1992 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN650234
507.14 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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