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Details for Product No. ABIN650245

ERK1 / ERK2 (pThr202), (pTyr204), (pThr185), (pTyr187) antibody

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Antigen
Epitope
»Alternatives pThr202, pTyr204, pThr185, pTyr187
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Pubmed 2 references available
Catalog no. ABIN650245
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Thr202 and Tyr204 of human ERK1 was used as immunogen. The antibody only detects ERK1 phosphorylated on Threonine 202 and Tyrosine 204, or ERK2 phosphorylated on Threonine 185 and Tyrosine 187.
Specificity A synthetic phospho-peptide corresponding to residues surrounding Thr202 and Tyr204 of human ERK1 was used as immunogen. The antibody only detects ERK1 phosphorylated on Threonine 202 and Tyrosine 204, or ERK2 phosphorylated on Threonine 185 and Tyrosine 187.
Background Extracellular signal-regulated kinases Erk1 (p44 MAPK) and Erk2 (p42 MAPK) Serine/Threonine protein kinase are part of a subfamily of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth, differentiation, apoptosis and signal transduction (1,2,3). The simultaneous tyrosine/threonine phosphorylation of ERK1 and ERK2 results in their activation. Although ERK1 and ERK2 resemble one another closely in sequence and are activated by similar pathways, they appear to have different tissue distributions (4) and show differential phosphorylation of potential intracellular substrates (5).
Synonyms: MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 3, p44-ERK1,ERT2,p44-MAPK,Microtubule-associated protein 2 kinase
Molecular Weight 42/44 kDA
Gene ID 5595
UniProt P27361
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:5000~10000 ICC: ~~ 1:250 IHC: ~~ 1:50
Comment

Background: Extracellular signal-regulated kinases Erk1 (p44 MAPK) and Erk2 (p42 MAPK) Serine/Threonine protein kinase are part of a subfamily of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth, differentiation, apoptosis and signal transduction (1,2,3). The simultaneous tyrosine/threonine phosphorylation of ERK1 and ERK2 results in their activation. Although ERK1 and ERK2 resemble one another closely in sequence and are activated by similar pathways, they appear to have different tissue distributions (4) and show differential phosphorylation of potential intracellular substrates (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Phospho Erk1 (pT202/pY204)/Erk2 (pT185/pY187) Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Charest, Mordret, Harder et al.: "Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4679-90, 1993 (PubMed).

Marshall: "Specificity of receptor tyrosine kinase signaling: transient versus sustained extracellular signal-regulated kinase activation." in: Cell, Vol. 80, Issue 2, pp. 179-85, 1995 (PubMed).

Alternatives for antigen "ERK1 / ERK2", type "Antibodies"
Hosts (108), (23), (4)
Reactivities (126), (68), (66), (44), (40), (36), (24), (12), (7), (4), (4), (1), (1)
Applications (100), (36), (30), (29), (25), (19), (16), (14), (6), (6), (2)
Conjugates (7), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Epitopes (44), (41), (19), (17), (12), (12), (6), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
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