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Erk1 /Erk2 (pThr202, pTyr204, pThr185, pTyr187) antibody
pThr202, pTyr204, pThr185, pTyr187
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
|2 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Thr202 and Tyr204 of human ERK1 was used as immunogen. The antibody only detects ERK1 phosphorylated on Threonine 202 and Tyrosine 204, or ERK2 phosphorylated on Threonine 185 and Tyrosine 187.|
|Description||Other names: MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 3, p44-ERK1,ERT2,p44-MAPK,Microtubule-associated protein 2 kinase|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic phospho-peptide corresponding to residues surrounding Thr202 and Tyr204 of human ERK1 was used as immunogen. The antibody only detects ERK1 phosphorylated on Threonine 202 and Tyrosine 204, or ERK2 phosphorylated on Threonine 185 and Tyrosine 187.|
|Molecular Weight||42/44 kDA|
Background: Extracellular signal-regulated kinases Erk1 (p44 MAPK) and Erk2 (p42 MAPK) Serine/Threonine protein kinase are part of a subfamily of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth, differentiation, apoptosis and signal transduction (1,2,3). The simultaneous tyrosine/threonine phosphorylation of ERK1 and ERK2 results in their activation. Although ERK1 and ERK2 resemble one another closely in sequence and are activated by similar pathways, they appear to have different tissue distributions (4) and show differential phosphorylation of potential intracellular substrates (5).
|Application Notes||The suggested dilution is: WB: ~~ 1:5000~10000 ICC: ~~ 1:250 IHC: ~~ 1:50|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Phospho Erk1 (pT202/pY204)/Erk2 (pT185/pY187) Antibody can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Charest, Mordret, Harder et al.: "Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4679-90, 1993 (PubMed).
Marshall: "Specificity of receptor tyrosine kinase signaling: transient versus sustained extracellular signal-regulated kinase activation." in: Cell, Vol. 80, Issue 2, pp. 179-85, 1995 (PubMed).