Protein Inhibitor of Activated STAT, 1 (PIAS1) (C-Term) antibody

Antigen: Protein Inhibitor of Activated STAT, 1 (PIAS1)

Synonyms: GBP, ZMIZ3, DDXBP1, GU/RH-II, MGC141878, MGC141879, pias1-A, xPAIS1, MGC82373

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650255

Additional Information

Alternative name: PIAS1

Gene ID: 8554

UniProt: O75925

Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human PIAS1 was used as an immunogen.

Description: Other names: PIAS1, DDXBP1, E3 SUMO-protein ligase PIAS1, RNA helicase II-binding protein,DEAD/H box-binding protein 1

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues on the C-terminus of human PIAS1 was used as an immunogen.

Molecular Weight: 78 kDA

Comments:

Background: PIAS1 (protein inhibitor of activated STAT) proteins blocks the DNA binding activity of Stat1 and inhibits Stat1-mediated gene activation in response to cytokine stimulation (1). PIAS1 also plays a crucial role as a transcriptional coregulator in the p53 pathway and the steroid hormone signaling pathway and functions as an E3 type small ubiquitin-like modifier (SUMO) ligas, stabilizing the interaction between UBEZ1 and the substrate, and as a SUMO-tethering factor. It is expressed in numerous tissues with highest level in testes.

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1: 1000~10000 ICC: ~~ 1: 100~250 IHC: ~~ 1: 100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: PIAS1 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Liu, Liao, Rao et al.: "Inhibition of Stat1-mediated gene activation by PIAS1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 18, pp. 10626-31, 1998 (PubMed).