Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) antibody

Antigen: Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4)

Synonyms: RP-C, PP2Ac, PP2CA, PP2A, R75353, Pp2a1, PPP2CA

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650312

Additional Information

Alternative name: PP2A

Gene ID: 5515

UniProt: P67775

Immunogen: A synthetic peptide corresponding to residues near C-terminus of human PP2A was used as immunogen. This antibody detects both alpha and beta isoforms of PP2A.

Description: Other names: PPP2CA, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, Replication protein C

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near C-terminus of human PP2A was used as immunogen. This antibody detects both alpha and beta isoforms of PP2A.

Molecular Weight: 36 kDA

Comments:

Background: Protein phosphatase type 2A (PP2A) is a protein serine/threonine phosphatase that controls fundamental cellular processes, including transcription, translation, metabolism, cell growth, apoptosis, and varied other signal transduction pathways (1-3). PP2A is comprised of a core enzyme, which is made up of catalytic C and regulatory A (PR65) subunits, and a secondary regulatory B subunit. This B subunit contains four different protein families. In response to EGF or insulin, it has been shown that Src phosphorylates PP2A at Tyr307, thereby inactivating PP2A (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:5000 IP: ~~ 1:100 ICC: ~~ 1:50 IHC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: PP2A Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Chromatin, Metabolism, Cell Structure

Restrictions: For Research Use only

Publications

Product: Janssens, Goris: "Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling." in: The Biochemical journal, Vol. 353, Issue Pt 3, pp. 417-39, 2001 (PubMed).