Pumilio Homolog 2 (Drosophila) (PUM2) antibody

Antigen: Pumilio Homolog 2 (Drosophila) (PUM2)

Synonyms: PUMH2, PUML2, FLJ36528, KIAA0235, MGC138251, MGC138253, pum-2

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650356

Additional Information

Alternative name: Pumilio-2

Gene ID: 9588

UniProt: Q8TB72

Immunogen: A synthetic peptide corresponding to residues in human Pumilio-2 was used as an immunogen. This antibody is predicted to not cross react with Pumilio-1.

Description: Other names: PUM1, KIAA0099, PUMH1, Pumilio homolog 1

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human Pumilio-2 was used as an immunogen. This antibody is predicted to not cross react with Pumilio-1.

Molecular Weight: 114 kDA

Comments:

Background: Pumilio-2 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. It belongs to the PUF family, 84% identical to Pumilio-1. It is required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts (1). Pumilio-2 is highly expressed in testis and ovary and is predominately expressed in stem cells and germ cells.

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500~1000 IP: ~~ 1:10 ICC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Pumilio-2 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: "The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).