RAD17 Homolog (S. Pombe) (RAD17) (pSer645) antibody

Details for Product No. ABIN650359
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms CCYC, HRAD17, R24L, RAD17SP, RAD24, 9430035O09Rik, MmRad24, K2A18.21, K2A18_21, RAD17, RADIATION SENSITIVE 17, zgc:56502, zgc:77779, xrad1, RAD1, DKFZp459H1127, zgc:91969
Epitope
pSer645
(21), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(92), (51), (36), (13), (13), (12), (3), (2), (1), (1)
Host
Rabbit
(91), (9), (6)
Clonality
Monoclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(88), (32), (20), (12), (11), (9), (6), (3), (3), (3), (1)
Pubmed 2 references available
Catalog no. ABIN650359
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A phospho-specific peptide corresponding to residues surrounding Serine 645 of human Rad17 was used as immunogen. The antibody only detects Rad17 phosphorylated on Serine 645.
Specificity A phospho-specific peptide corresponding to residues surrounding Serine 645 of human Rad17 was used as immunogen. The antibody only detects Rad17 phosphorylated on Serine 645.
Alternative Name RAD17
Background Checkpoint Rad proteins function early in the DNA damage signaling cascade to arrest cell cycle progression in response to DNA damage (1). There is a direct regulatory linkage between the Rad17 homologue and the checkpoint kinases, ATM and ATR (2). ATR but not ATM phosphorylates the Rad17 checkpoint protein on Ser635 and Ser645 in vitro. In undamaged synchronized human cells, these two sites were phosphorylated in late G(1), S, and G(2)/M, but not in early-mid G(1). Treatment of cells with genotoxic stress induced phosphorylation of Rad17 in cells in early-mid G(1). This suggests that ATR and Rad17 are essential components of a DNA damage response pathway in mammalian cells (3).
Synonyms: RAD17, R24L, Cell cycle checkpoint protein RAD17, RF-C/activator 1 homolog
Molecular Weight 80 kDA
Gene ID 5884
UniProt O75943
Research Area Phospho-specific antibodies, Protein Modifications, Signaling, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:2500-5000
Comment

Background: Checkpoint Rad proteins function early in the DNA damage signaling cascade to arrest cell cycle progression in response to DNA damage (1). There is a direct regulatory linkage between the Rad17 homologue and the checkpoint kinases, ATM and ATR (2). ATR but not ATM phosphorylates the Rad17 checkpoint protein on Ser635 and Ser645 in vitro. In undamaged synchronized human cells, these two sites were phosphorylated in late G(1), S, and G(2)/M, but not in early-mid G(1). Treatment of cells with genotoxic stress induced phosphorylation of Rad17 in cells in early-mid G(1). This suggests that ATR and Rad17 are essential components of a DNA damage response pathway in mammalian cells (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Rad17 Antibody Phospho (pS645) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Bao, Tibbetts, Brumbaugh et al.: "ATR/ATM-mediated phosphorylation of human Rad17 is required for genotoxic stress responses." in: Nature, Vol. 411, Issue 6840, pp. 969-74, 2001 (PubMed).

Lindsey-Boltz, Bermudez, Hurwitz et al.: "Purification and characterization of human DNA damage checkpoint Rad complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11236-41, 2001 (PubMed).

Hosts (91), (9), (6)
Reactivities (92), (51), (36), (13), (13), (12), (3), (2), (1), (1)
Applications (88), (32), (20), (12), (11), (9), (6), (3), (3), (3), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (21), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top