RAD17 Homolog (S. Pombe) (RAD17) (pSer645) antibody

Antigen: RAD17 Homolog (S. Pombe) (RAD17)

Synonyms: CCYC, R24L, RAD24, HRAD17, RAD17SP, FLJ41520, MGC77779, zgc:56502, zgc:77779, RAD1, rad1, RAD17, DKFZp459H1127, zgc:91969, MGC140462

Binding Site: pSer645

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN650359

Additional Information

Alternative name: RAD17

Gene ID: 5884

UniProt: O75943

Immunogen: A phospho-specific peptide corresponding to residues surrounding Serine 645 of human Rad17 was used as immunogen. The antibody only detects Rad17 phosphorylated on Serine 645.

Description: Other names: RAD17, R24L, Cell cycle checkpoint protein RAD17, RF-C/activator 1 homolog

Characteristics: Rabbit Monoclonal Antibody

Specificity: A phospho-specific peptide corresponding to residues surrounding Serine 645 of human Rad17 was used as immunogen. The antibody only detects Rad17 phosphorylated on Serine 645.

Molecular Weight: 80 kDA

Comments:

Background: Checkpoint Rad proteins function early in the DNA damage signaling cascade to arrest cell cycle progression in response to DNA damage (1). There is a direct regulatory linkage between the Rad17 homologue and the checkpoint kinases, ATM and ATR (2). ATR but not ATM phosphorylates the Rad17 checkpoint protein on Ser635 and Ser645 in vitro. In undamaged synchronized human cells, these two sites were phosphorylated in late G(1), S, and G(2)/M, but not in early-mid G(1). Treatment of cells with genotoxic stress induced phosphorylation of Rad17 in cells in early-mid G(1). This suggests that ATR and Rad17 are essential components of a DNA damage response pathway in mammalian cells (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:2500~5000

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Rad17 Antibody Phospho (pS645) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Protein Modifications, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Bao, Tibbetts, Brumbaugh et al.: "ATR/ATM-mediated phosphorylation of human Rad17 is required for genotoxic stress responses." in: Nature, Vol. 411, Issue 6840, pp. 969-74, 2001 (PubMed).

Lindsey-Boltz, Bermudez, Hurwitz et al.: "Purification and characterization of human DNA damage checkpoint Rad complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11236-41, 2001 (PubMed).