Did you know that you can buy products from over 140 different suppliers from us?

V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (Ser338-339) antibody

Details for Product No. ABIN650362
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms c-raf, craf, raf1, RAF1, raf, CRAF, NS5, Raf-1, c-Raf, 6430402F14Rik, AA990557, BB129353, Craf1, D830050J10Rik, v-Raf, AFR1, RAF, Afr-1, Afr1, Raf, mKIAA0854
Epitope
Ser338-339
(63), (45), (31), (30), (25), (24), (24), (24), (24), (21), (20), (17), (17), (17), (16), (16), (14), (13), (12), (12), (8), (8), (7), (7), (7), (6), (6), (6), (6), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(608), (331), (323), (151), (149), (120), (113), (12), (2), (2), (2), (1)
Host
Rabbit
(566), (33), (15), (8), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(28), (28), (28), (15), (15), (15), (13), (13), (13), (13), (13), (13), (13), (13)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(383), (267), (141), (130), (102), (95), (27), (16), (13), (11), (2), (2), (1), (1)
Pubmed 2 references available
Quantity 100 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN650362
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A synthetic peptide corresponding to residues surrounding Serine 338-339 of human Raf was used as immunogen.
Specificity A synthetic peptide corresponding to residues surrounding Serine 338-339 of human Raf was used as immunogen.
Alternative Name Raf-1
Background Raf-1 (c-Raf) is a protein kinase that is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Raf-1 is recruited by and binds to GTP-bound Ras, activating the MEK-MAP kinase pathway (1). Raf-1 is activated via rapid hyperphosphorylation at multiple sites in mammalian cells, including Ser43, Ser259, and Ser621 (2), as well as Ser338, Tyr341, Thr491, and Ser494 (3). p21-activated protein kinase (PAK) phosphorylates Raf-1 at Ser338 in response to Ras, Src and epidermal growth factor (4).
Synonyms: RAF1, RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1
Molecular Weight 74 kDA
Gene ID 5894
UniProt P04049
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Signaling, Chromatin, Cell Structure
Application Notes IHC: = 1:50-100, WB: = 1:1000-20000
Comment

Background: Raf-1 (c-Raf) is a protein kinase that is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Raf-1 is recruited by and binds to GTP-bound Ras, activating the MEK-MAP kinase pathway (1). Raf-1 is activated via rapid hyperphosphorylation at multiple sites in mammalian cells, including Ser43, Ser259, and Ser621 (2), as well as Ser338, Tyr341, Thr491, and Ser494 (3). p21-activated protein kinase (PAK) phosphorylates Raf-1 at Ser338 in response to Ras, Src and epidermal growth factor (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Raf-1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications King, Sun, Diaz et al.: "The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338." in: Nature, Vol. 396, Issue 6707, pp. 180-3, 1998 (PubMed).

Chong, Lee, Guan: "Positive and negative regulation of Raf kinase activity and function by phosphorylation." in: The EMBO journal, Vol. 20, Issue 14, pp. 3716-27, 2001 (PubMed).

Validation Images
back to top