Ran GTPase Activating Protein 1 (RANGAP1) antibody

Details for Product No. ABIN650366
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Antigen
Reactivity
Human, Rat (Rattus)
(75), (31), (24), (6), (4), (4), (1), (1), (1)
Host
Rabbit
(54), (17), (8), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(65), (31), (30), (24), (19), (10), (4), (3), (3), (3), (2), (2)
Pubmed 3 references available
Quantity 100 μL
Options
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Request Want additional data for this product?

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Immunogen A synthetic peptide corresponding to residues in human RanGAP1 was used as an immunogen.
Specificity A synthetic peptide corresponding to residues in human RanGAP1 was used as an immunogen.
Alternative Name RanGAP1 (RANGAP1 Antibody Abstract)
Background RanGAP1 is the activator of the Ras-related nuclear GTPase Ran, which is involved in the nucleo-cytoplasmic transport, mitotic spindle assembly, cell cycle control and nuclear envelope (NE) formation (1, 2). Its roles are accomplished by the asymmetric distribution of its GTP- and GDP-bound forms, enabled by the specific localization of Ran accessory proteins, the Ran GTPase-activating protein RanGAP and the nucleotide exchange factor RCC. Mammalian RanGAP1 is targeted to the NE during interphase and to the spindle and kinetochores during mitosis via SUMOylated C-terminal domain and interaction with the nucleoporin Nup358/RanBP2 (2). RanGAP1 is highly expressed in brain, thymus and testis (3).
Molecular Weight 90/70 kDA
Gene ID 4879
UniProt P46060
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure, Chromatin
Application Notes The suggested dilution is: WB: = 1:2000-5000 , IHC: = 1:100-250
Comment

Background: RanGAP1 is the activator of the Ras-related nuclear GTPase Ran, which is involved in the nucleo-cytoplasmic transport, mitotic spindle assembly, cell cycle control and nuclear envelope (NE) formation (1, 2). Its roles are accomplished by the asymmetric distribution of its GTP- and GDP-bound forms, enabled by the specific localization of Ran accessory proteins, the Ran GTPase-activating protein RanGAP and the nucleotide exchange factor RCC. Mammalian RanGAP1 is targeted to the NE during interphase and to the spindle and kinetochores during mitosis via SUMOylated C-terminal domain and interaction with the nucleoporin Nup358/RanBP2 (2). RanGAP1 is highly expressed in brain, thymus and testis (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment RanGAP1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Meier, Xu, Brkljacic et al.: "Going green: plants' alternative way to position the Ran gradient." in: Journal of microscopy, Vol. 231, Issue 2, pp. 225-33, 2008 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).

Krebber, Ponstingl: "Ubiquitous expression and testis-specific alternative polyadenylation of mRNA for the human Ran GTPase activator RanGAP1." in: Gene, Vol. 180, Issue 1-2, pp. 7-11, 1997 (PubMed).

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Catalog No. ABIN650366
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