Ran GTPase Activating Protein 1 (RANGAP1) antibody

Antigen: Ran GTPase Activating Protein 1 (RANGAP1)

Synonyms: SD, Fug1, KIAA1835, MGC20266, RAN GTPASE ACTIVATING PROTEIN 1, RANGAP1, fug1, rna1p, RanGAP1, rangap1b, MGC154793

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650366

Additional Information

Alternative name: RanGAP1

Gene ID: 4879

UniProt: P46060

Immunogen: A synthetic peptide corresponding to residues in human RanGAP1 was used as an immunogen.

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human RanGAP1 was used as an immunogen.

Molecular Weight: 90/70 kDA

Comments:

Background: RanGAP1 is the activator of the Ras-related nuclear GTPase Ran, which is involved in the nucleo-cytoplasmic transport, mitotic spindle assembly, cell cycle control and nuclear envelope (NE) formation (1, 2). Its roles are accomplished by the asymmetric distribution of its GTP- and GDP-bound forms, enabled by the specific localization of Ran accessory proteins, the Ran GTPase-activating protein RanGAP and the nucleotide exchange factor RCC. Mammalian RanGAP1 is targeted to the NE during interphase and to the spindle and kinetochores during mitosis via SUMOylated C-terminal domain and interaction with the nucleoporin Nup358/RanBP2 (2). RanGAP1 is highly expressed in brain, thymus and testis (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:2000~5000 IP: ~~ 1:10 FC: ~~ 1:50 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: RanGAP1 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure, Chromatin

Restrictions: For Research Use only

Publications

Product: Krebber, Ponstingl: "Ubiquitous expression and testis-specific alternative polyadenylation of mRNA for the human Ran GTPase activator RanGAP1." in: Gene, Vol. 180, Issue 1-2, pp. 7-11, 1997 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).

Meier, Xu, Brkljacic et al.: "Going green: plants' alternative way to position the Ran gradient." in: Journal of microscopy, Vol. 231, Issue 2, pp. 225-33, 2008 (PubMed).