RAP1 antibody

Antigen: RAP1

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN650367

Additional Information

Gene ID: 5906

UniProt: P62834

Immunogen: A synthetic peptide corresponding to residues near the N-terminus of human Rap1 was used as an immunogen.

Description: Other names: RAP1A, KREV1, Ras-related protein Rap-1A, GTP-binding protein smg-p21A,Ras-related protein Krev-1,C21KG,G-22K

Characteristics: Rabbit Polyclonal IgG Affinity Purified

Specificity: A synthetic peptide corresponding to residues near the N-terminus of human Rap1 was used as an immunogen.

Molecular Weight: 21 kDA

Comments:

Background: Rap1, also known as Krev-1 and smg p21, is a member of the Ras super family of GTPases closely related to Ras. Two known isoforms of Rap1 exists, Rap1A and Rap1B which share 95% identity (1-2). As a molecular switch, Rap1 cycles between two conformational states depending on whether GDP bound (active form) or GTP bound (inactive form). Rap1 associates with a subset of Ras effectors including Raf-1, B-Raf, phosphoinositide 3-kinase, Ral GEFs, and PLC (3). Although its function is still unclear, Rap1 has been shown to be involved in the regulation of integrin-mediated cell adhesion (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500 FC: ~~ 1:20 IHC: ~~ 1:50~100

Purification: Purified

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Rap1 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Protein Modifications, Cell Structure, Cancer, Chromatin

Restrictions: For Research Use only

Publications

Product: Rousseau-Merck, Pizon, Tavitian et al.: "Chromosome mapping of the human RAS-related RAP1A, RAP1B, and RAP2 genes to chromosomes 1p12----p13, 12q14, and 13q34, respectively." in: Cytogenetics and cell genetics, Vol. 53, Issue 1, pp. 2-4, 1990 (PubMed).

Kitayama, Sugimoto, Matsuzaki et al.: "A ras-related gene with transformation suppressor activity." in: Cell, Vol. 56, Issue 1, pp. 77-84, 1989 (PubMed).