Retinoblastoma 1 (RB1) antibody

Details for Product No. ABIN650375
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Antigen
Synonyms RB1, OSRC, RB, p105-Rb, pRb, pp110, Rb, Rb-1, zgc:154147, P105-RB, PP110, RB11
Reactivity
Human
(339), (227), (215), (109), (85), (65), (48), (7), (1), (1), (1)
Host
Rabbit
(298), (54), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10)
Application
Western Blotting (WB)
(212), (100), (94), (88), (80), (34), (31), (18), (11), (7), (2), (1)
Pubmed 3 references available
Quantity 100 µL
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Catalog No. ABIN650375
392.86 $
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Immunogen A synthetic peptide corresponding to residues in human Rb was used as an immunogen.
Specificity A synthetic peptide corresponding to residues in human Rb was used as an immunogen.
Alternative Name Rb
Background Retinoblastoma tumor suppressor protein (Rb) is associated with DNA binding activity and is thought to play a significant role in controlling cell cycle progression during tumor growth (1, 2). Cell cycle-dependent phosphorylation by cdk's inhibits Rb binding, thus allowing cell cycle progression (3). Rb inactivation and cell cycle progression likely requires first phosphorylation by cyclin D-cdk4/6 followed by cyclin E-cdk2 phosphorylation (4).
Synonyms: RB1, Retinoblastoma-associated protein, pp110,p105-Rb
Molecular Weight 110 kDA
Gene ID 5925
UniProt P06400
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-2000
Comment

Background: Retinoblastoma tumor suppressor protein (Rb) is associated with DNA binding activity and is thought to play a significant role in controlling cell cycle progression during tumor growth (1, 2). Cell cycle-dependent phosphorylation by cdk's inhibits Rb binding, thus allowing cell cycle progression (3). Rb inactivation and cell cycle progression likely requires first phosphorylation by cyclin D-cdk4/6 followed by cyclin E-cdk2 phosphorylation (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Rb Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Sherr: "Cancer cell cycles." in: Science (New York, N.Y.), Vol. 274, Issue 5293, pp. 1672-7, 1997 (PubMed).

Knudsen, Wang: "Dual mechanisms for the inhibition of E2F binding to RB by cyclin-dependent kinase-mediated RB phosphorylation." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 5771-83, 1997 (PubMed).

Lundberg, Weinberg: "Functional inactivation of the retinoblastoma protein requires sequential modification by at least two distinct cyclin-cdk complexes." in: Molecular and cellular biology, Vol. 18, Issue 2, pp. 753-61, 1998 (PubMed).

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