|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Retinoblastoma 1 (RB1) antibody
|Synonyms||RB, pRb, OSRC, pp110, p105-Rb, Rb, Rb-1, RB1, RB11, PP110, P105-RB|
Alternatives Western Blotting (WB)
|3 references available|
|Price||392.86 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||A synthetic peptide corresponding to residues in human Rb was used as an immunogen.|
|Description||Other names: RB1, Retinoblastoma-associated protein, pp110,p105-Rb|
|Specificity||A synthetic peptide corresponding to residues in human Rb was used as an immunogen.|
|Molecular Weight||110 kDA|
Background: Retinoblastoma tumor suppressor protein (Rb) is associated with DNA binding activity and is thought to play a significant role in controlling cell cycle progression during tumor growth (1, 2). Cell cycle-dependent phosphorylation by cdk's inhibits Rb binding, thus allowing cell cycle progression (3). Rb inactivation and cell cycle progression likely requires first phosphorylation by cyclin D-cdk4/6 followed by cyclin E-cdk2 phosphorylation (4).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000~2000|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Rb Antibody can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Sherr: "Cancer cell cycles." in: Science (New York, N.Y.), Vol. 274, Issue 5293, pp. 1672-7, 1997 (PubMed).
Knudsen, Wang: "Dual mechanisms for the inhibition of E2F binding to RB by cyclin-dependent kinase-mediated RB phosphorylation." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 5771-83, 1997 (PubMed).
Lundberg, Weinberg: "Functional inactivation of the retinoblastoma protein requires sequential modification by at least two distinct cyclin-cdk complexes." in: Molecular and cellular biology, Vol. 18, Issue 2, pp. 753-61, 1998 (PubMed).