|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Retinoblastoma 1 (RB1) (pSer780) antibody
|Synonyms||RB, pRb, OSRC, pp110, p105-Rb, Rb, Rb-1, RB1, RB11, PP110, P105-RB|
Alternatives Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
|2 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Ser780 of human Rb was used as immunogen. The antibody only detects Rb phosphorylated on Serine 780.|
|Description||Other names: RB1, Retinoblastoma-associated protein, pp110,p105-Rb|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic phospho-peptide corresponding to residues surrounding Ser780 of human Rb was used as immunogen. The antibody only detects Rb phosphorylated on Serine 780.|
|Molecular Weight||110 kDA|
Background: Retinoblastoma tumor suppressor protein (Rb) is associated with DNA binding activity and is thought to play a significant role in controlling cell cycle progression during tumor growth (1,2). Cell cycle-dependent phosphorylation by cdk',s inhibits Rb binding, thus allowing cell cycle progression (3). Rb inactivation and cell cycle progression likely requires first phosphorylation by cyclin D-cdk4/6 followed by cyclin E-cdk2 phosphorylation (4).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000 IP: ~~ 1:50 ICC: ~~ 1:250~1:500|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Rb Antibody Phospho (pS780) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Lee, Shew, Hong et al.: "The retinoblastoma susceptibility gene encodes a nuclear phosphoprotein associated with DNA binding activity." in: Nature, Vol. 329, Issue 6140, pp. 642-5, 1987 (PubMed).
Sherr: "Cancer cell cycles." in: Science (New York, N.Y.), Vol. 274, Issue 5293, pp. 1672-7, 1997 (PubMed).