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Retinoblastoma 1 (RB1) (pThr821) antibody

Antigen

Retinoblastoma 1 (RB1)
Synonyms RB, pRb, OSRC, pp110, p105-Rb, Rb, Rb-1, RB1, RB11, PP110, P105-RB
Binding Site
Alternatives

pThr821
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN650379
Quantity 100ul
Price 507.14 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Rb
Gene ID 5925
UniProt P06400
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Thr821 of human Rb was used as immunogen. The antibody only detects Rb phosphorylated on Threonine 821.
Description Other names: RB1, Retinoblastoma-associated protein, pp110,p105-Rb
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic phospho-peptide corresponding to residues surrounding Thr821 of human Rb was used as immunogen. The antibody only detects Rb phosphorylated on Threonine 821.
Molecular Weight 110 kDA
Comments

Background: Retinoblastoma tumor suppressor protein (Rb) is associated with DNA binding activity and is thought to play a significant role in controlling cell cycle progression during tumor growth (1,2). Cell cycle-dependent phosphorylation by cdks inhibits Rb binding, thus allowing cell cycle progression (3). Rb inactivation and cell cycle progression likely requires first phosphorylation by cyclin D-cdk4/6 followed by cyclin E-cdk2 phosphorylation (4).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:20 ICC: ~~ 1:100
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Rb Antibody Phospho (pT821) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Chromatin, Cell Structure
Restrictions For Research Use only

Publications
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Product Lee, Shew, Hong et al.: "The retinoblastoma susceptibility gene encodes a nuclear phosphoprotein associated with DNA binding activity." in: Nature, Vol. 329, Issue 6140, pp. 642-5, 1987 (PubMed).

Sherr: "Cancer cell cycles." in: Science (New York, N.Y.), Vol. 274, Issue 5293, pp. 1672-7, 1997 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Retinoblastoma 1 (RB1)", type "Antibodies"
Hosts Rabbit (215), Mouse (68), Rat (1)
Reactivities Human (269), Rat (Rattus) (111), Mouse (Murine) (105), Cow (Bovine) (40), Horse (Equine) (33), Pig (Porcine) (29), Dog (Canine) (17), Chicken (15), Mink (1), Rabbit (1), Zebrafish (1)
Applications Western Blotting (WB) (175), Immunofluorescence (IF) (90), ELISA (76), Immunohistochemistry (IHC) (76), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (62), Immunoprecipitation (IP) (42), Flow Cytometry (FACS) (10), Dot Blot (Dot) (5), Immunocytochemistry (ICC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (10), Alexa Fluor 488 (10), Alexa Fluor 555 (10), Alexa Fluor 647 (10), PE,Cy5.5 (10), Biotin (5), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer780 (22), pSer795 (18), pSer807 (12), C-Term (9), pSer608 (9), pSer612 (9), pSer788 (8), pSer249 (7), pThr826 (7), pThr821 (6), AA 393-572 (5), pSer811 (5), pSer811,pSer807 (4), Internal Region (3), N-Term (3), Ser780 (3), pThr356 (3), Ser608 (2), Ser795 (2), Ser807 (2), AA 121-140 (1), AA 251-268 (1), AA 280-300 (1), AA 371-390 (1), AA 41-330 (1), AA 511-530 (1), AA 571-590 (1), AA 612-928 (1), AA 800-850 (1), AA 886-905 (1), Center,Ser249 (1), pSer1106 (1), pSer249,pThr252 (1), pSer338 (1), pSer339 (1), pSer807,pSer811 (1), pSer8111 (1), pSer821 (1), pSer826 (1), pThr252,pSer249 (1), pThr373 (1)