Retinoblastoma-Like 2 (p130) (RBL2) (pSer952) antibody

Details for Product No. ABIN650382
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Antigen
Synonyms RBL2, RBR2, RRB2a, PRB2, RBR-2, Rb2, p130, P130
Epitope
pSer952
(17), (10), (8), (5), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(102), (48), (48), (37), (37), (23), (3), (2), (2), (2), (2)
Host
Rabbit
(81), (24)
Clonality
Monoclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(60), (37), (29), (28), (24), (12), (9), (8), (4), (3)
Pubmed 3 references available
Quantity 100 μL
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Catalog No. ABIN650382
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Immunogen A synthetic phosphor peptide corresponding to residues surrounding serine 952 of human p130 was used as an immunogen.
Specificity A synthetic phosphor peptide corresponding to residues surrounding serine 952 of human p130 was used as an immunogen.
Alternative Name Rb2 (p130)
Background P130 is a tumor suppressor of the pocket protein family whose expression is posttranscriptionally regulated and largely G0 restricted. The mechanism of down-regulation of p130 expression in proliferating cells was investigated and results indicate that the decline of p130 expression as G0 cells reenter the cell cycle is due to a decrease in protein stability. In vitro polyubiquitination of p130 by SCF(Skp2) was specifically dependent on phosphorylation of p130 on Serine 672 (1). The retinoblastoma family of proteins including pRB, p107 and p130 undergoes cell cycle dependent phosphorylation during the mid-G1 to S phase transition. This phosphorylation is dependent upon the activity of cyclin D/cdk4. In contrast to pRB and p107, p130 is phosphorylated during G0 and the early G1 phase of the cell cycle. Also it is thought that p130, forming a complex with Rad50 through RINT-1, blocks telomerase-independent telomere lengthening in normal cells. Data indicates complementary roles for the Rb/E2F pathway in the control of telomere length (3).
Synonyms: RBL2, RB2, Retinoblastoma-like protein 2, PRB2,130 kDa retinoblastoma-associated protein,p130
Molecular Weight 130 kDA
Gene ID 5934
UniProt Q08999
Research Area Translation Factors, Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Chromatin, Signaling, Cell Structure
Application Notes IHC: = 1:100-250, WB: = 1:1000-10000
Comment

Background: p130 is a tumor suppressor of the pocket protein family whose expression is posttranscriptionally regulated and largely G0 restricted. The mechanism of down-regulation of p130 expression in proliferating cells was investigated and results indicate that the decline of p130 expression as G0 cells reenter the cell cycle is due to a decrease in protein stability. In vitro polyubiquitination of p130 by SCF(Skp2) was specifically dependent on phosphorylation of p130 on Serine 672 (1). The retinoblastoma family of proteins including pRB, p107 and p130 undergoes cell cycle dependent phosphorylation during the mid-G1 to S phase transition. This phosphorylation is dependent upon the activity of cyclin D/cdk4. In contrast to pRB and p107, p130 is phosphorylated during G0 and the early G1 phase of the cell cycle. Also it is thought that p130, forming a complex with Rad50 through RINT-1, blocks telomerase-independent telomere lengthening in normal cells. Data indicates complementary roles for the Rb/E2F pathway in the control of telomere length (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Rb2 (p130) Antibody Phospho (pS952) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Canhoto, Chestukhin, Litovchick et al.: "Phosphorylation of the retinoblastoma-related protein p130 in growth-arrested cells." in: Oncogene, Vol. 19, Issue 44, pp. 5116-22, 2000 (PubMed).

Tedesco, Lukas, Reed: "The pRb-related protein p130 is regulated by phosphorylation-dependent proteolysis via the protein-ubiquitin ligase SCF(Skp2)." in: Genes & development, Vol. 16, Issue 22, pp. 2946-57, 2002 (PubMed).

Kong, Meloni, Nevins: "The Rb-related p130 protein controls telomere lengthening through an interaction with a Rad50-interacting protein, RINT-1." in: Molecular cell, Vol. 22, Issue 1, pp. 63-71, 2006 (PubMed).

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