Retinoblastoma-Like 2 (p130) (RBL2) (pSer952) antibody

Antigen: Retinoblastoma-Like 2 (p130) (RBL2)

Synonyms: RBL2, MGC154964

Binding Site: pSer952

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN650382

Additional Information

Alternative name: Rb2 (p130)

Gene ID: 5934

UniProt: Q08999

Immunogen: A synthetic phosphor peptide corresponding to residues surrounding serine 952 of human p130 was used as an immunogen.

Description: Other names: RBL2, RB2, Retinoblastoma-like protein 2, PRB2,130 kDa retinoblastoma-associated protein,p130

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic phosphor peptide corresponding to residues surrounding serine 952 of human p130 was used as an immunogen.

Molecular Weight: 130 kDA

Comments:

Background: p130 is a tumor suppressor of the pocket protein family whose expression is posttranscriptionally regulated and largely G0 restricted. The mechanism of down-regulation of p130 expression in proliferating cells was investigated and results indicate that the decline of p130 expression as G0 cells reenter the cell cycle is due to a decrease in protein stability. In vitro polyubiquitination of p130 by SCF(Skp2) was specifically dependent on phosphorylation of p130 on Serine 672 (1). The retinoblastoma family of proteins including pRB, p107 and p130 undergoes cell cycle dependent phosphorylation during the mid-G1 to S phase transition. This phosphorylation is dependent upon the activity of cyclin D/cdk4. In contrast to pRB and p107, p130 is phosphorylated during G0 and the early G1 phase of the cell cycle. Also it is thought that p130, forming a complex with Rad50 through RINT-1, blocks telomerase-independent telomere lengthening in normal cells. Data indicates complementary roles for the Rb/E2F pathway in the control of telomere length (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Rb2 (p130) Antibody Phospho (pS952) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Translation Factors, Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Canhoto, Chestukhin, Litovchick et al.: "Phosphorylation of the retinoblastoma-related protein p130 in growth-arrested cells." in: Oncogene, Vol. 19, Issue 44, pp. 5116-22, 2000 (PubMed).

Tedesco, Lukas, Reed: "The pRb-related protein p130 is regulated by phosphorylation-dependent proteolysis via the protein-ubiquitin ligase SCF(Skp2)." in: Genes & development, Vol. 16, Issue 22, pp. 2946-57, 2002 (PubMed).

Kong, Meloni, Nevins: "The Rb-related p130 protein controls telomere lengthening through an interaction with a Rad50-interacting protein, RINT-1." in: Molecular cell, Vol. 22, Issue 1, pp. 63-71, 2006 (PubMed).