Retinoblastoma Binding Protein 4 (RBBP4) antibody

Antigen: Retinoblastoma Binding Protein 4 (RBBP4)

Synonyms: NURF55, RBAP48, 154659_at, 55, caf-1, Caf-1, CAF-1, caf1, CAF1, caf1 p55, Caf1/p55, Caf1p55, CAF1p55, CG4236, d-CAF1, dCAF-1, dCAF-1 p55, DmelCG4236, dNURF, MSI1/RbAp48/CAC3/LIN-53, NURF, Nurf-55, NURF-55, Nurf55, p55, P55, p55/NURF-55, p55CAF1, RbAp48, nurf55, rbap48, MGC79922, mRbAp48, RBBP4, rbb4-2, zgc:55349, zgc:77854, wu:fb33a09, wu:fb40e10, MGC128413, RBBP-4, DKFZp469A0122

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650383

Additional Information

Alternative name: RBBP4

Gene ID: 5928

UniProt: Q09028

Immunogen: A synthetic peptide corresponding to residues on the N-term of human RBBP4 was used as an immunogen.

Description: Other names: RBBP4, RBAP48, Histone-binding protein RBBP4, Chromatin assembly factor I p48 subunit,CAF-I 48 kDa subunit,CAF-I p48,Nucleosome-remodeling factor subunit RBAP48

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues on the N-term of human RBBP4 was used as an immunogen.

Molecular Weight: 52 kDA

Comments:

Background: RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. RBBP4 is a component of several complexes which regulate chromatin metabolism. These complexes include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair as well as the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression (1) The growth suppression function of the retinoblastoma protein (Rb) is thought to be mediated by Rb binding to cellular proteins. Rb interacts with multiple cellular proteins that mediate its cellular function. p48 is one of the major proteins that binds to a putative functional domain at the carboxy terminus of the Rb protein (2). Interaction with p48 may be one of the mechanisms for suppression of growth mediated by Rb. RbAp48 (RBBP4) and RbAp46 are quantitatively the major Rb-associated proteins. Results suggest that RBBP4 and RbAp46 may have shared as well as unique functions in the regulation of cell proliferation and differentiation (3).

Synonyms: NURF55, RBAP48, 154659_at, 55, caf-1, Caf-1, CAF-1, caf1, CAF1, caf1 p55, Caf1/p55, Caf1p55, CAF1p55, CG4236, d-CAF1, dCAF-1, dCAF-1 p55, DmelCG4236, dNURF, MSI1/RbAp48/CAC3/LIN-53, NURF, Nurf-55, NURF-55, Nurf55, p55, P55, p55/NURF-55, p55CAF1, RbAp48, nurf55, rbap48, MGC79922, mRbAp48, RBBP4, rbb4-2, zgc:55349, zgc:77854, wu:fb33a09, wu:fb40e10, MGC128413, RBBP-4, DKFZp469A0122

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500~5000 FC: ~~ 1:55 ICC: ~~ 1: 100~250 IHC: ~~ 1: 100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: RBBP4 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Qian, Lee: "Dual retinoblastoma-binding proteins with properties related to a negative regulator of ras in yeast." in: The Journal of biological chemistry, Vol. 270, Issue 43, pp. 25507-13, 1995 (PubMed).

Qian, Wang, Hollingsworth et al.: "A retinoblastoma-binding protein related to a negative regulator of Ras in yeast." in: Nature, Vol. 364, Issue 6438, pp. 648-52, 1993 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).