Ras Homolog Enriched in Brain (RHEB) antibody

Antigen: Ras Homolog Enriched in Brain (RHEB)

Synonyms: RHEB2, MGC111559, RHEB, rheb2, MGC53194, MGC153231, im:7161941, zgc:153231

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN650388

Additional Information

Alternative name: Rheb

Gene ID: 6009

UniProt: Q15382

Immunogen: A synthetic peptide corresponding to residues in human Rheb was used as an immunogen.

Description: Other names: RHEB, RHEB2, GTP-binding protein Rheb, Ras homolog enriched in brain

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human Rheb was used as an immunogen.

Molecular Weight: 21 kDA

Comments:

Background: Rheb (Ras Homolog Enriched in brain) is a Ras-like small guanosine triphosphatase (GTPase) that is involved in cell growth and cell cycle progression. Rheb has also been suggested to have involvement in the TOR/S6K signaling pathway (1). It regulates the activity of the mammalian target of rapamycin, mTOR, a central regulator of cell growth, in response to growth factor stimulation and nutrient availability (2). It also interacts with and appears to regulate Raf-1 kinase, an essential component of the H-Ras signaling pathway, and is suggested to function in concert with H-Ras to dynamically integrate cAMP and growth factor signaling (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:5000~10000 FC: ~~ 1:100 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Rheb Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Protein Modifications, Cell Structure, Chromatin

Restrictions: For Research Use only

Publications

Product: Tabancay, Gau, Machado et al.: "Identification of dominant negative mutants of Rheb GTPase and their use to implicate the involvement of human Rheb in the activation of p70S6K." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 39921-30, 2003 (PubMed).

Hsu, Chern, Cai et al.: "Drosophila TCTP is essential for growth and proliferation through regulation of dRheb GTPase." in: Nature, Vol. 445, Issue 7129, pp. 785-8, 2007 (PubMed).

Bai, Ma, Liu et al.: "Rheb activates mTOR by antagonizing its endogenous inhibitor, FKBP38." in: Science (New York, N.Y.), Vol. 318, Issue 5852, pp. 977-80, 2007 (PubMed).