rho (RHO) antibody

Antigen: rho (RHO)

Synonyms: ARHA, ARH12, RHO12, RHOH12, Arha, Arha1, Arha2, arha, arha2, xrhoa, MGC53405, arh12, rho12, rhoh12, MGC69542

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN650389

Additional Information

Alternative name: Rho

Gene ID: 387

UniProt: P61586

Immunogen: A synthetic peptide corresponding to residues near the N-term of human Rho was used as immunogen. The antibody is predicted to detect RhoA, RhoB and RhoC based on sequence analysis.

Description: Other names: RHOA, ARH12, ARHA, RHO12, Transforming protein RhoA, H12

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the N-term of human Rho was used as immunogen. The antibody is predicted to detect RhoA, RhoB and RhoC based on sequence analysis.

Molecular Weight: 22 kDA

Comments:

Background: Rho belongs to the Ras super family of low molecular weight GTPases. Fifteen Rho proteins have already been characterized and divided into three sub-families, the first includes Rho (A, B, C), the second, Rnd 1-3 and the third which includes Rac 1-3, RhoG, Cdc42Hs, Rho/TTF. TC10 and Chp (1-2). Rho acts as a molecular switch which turns on of off various intracellular signaling pathways such as ACK, PAKs, MEKKs ROCK (3). Rho is active when bound to GTP and inactive when bound to GDP (4). It is also known to participate in many physiological activities including cell migration, adhesion, cytokinesis, proliferation, differentiation and apoptosis and to a greater extend cell transformation (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:5000~50000 ICC: ~~ 1:250~500 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Rho Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure, Cancer, Chromatin

Restrictions: For Research Use only

Publications

Product: Yeramian, Chardin, Madaule et al.: "Nucleotide sequence of human rho cDNA clone 12." in: Nucleic acids research, Vol. 15, Issue 4, pp. 1869, 1987 (PubMed).

Khosravi-Far, Solski, Clark et al.: "Activation of Rac1, RhoA, and mitogen-activated protein kinases is required for Ras transformation." in: Molecular and cellular biology, Vol. 15, Issue 11, pp. 6443-53, 1995 (PubMed).

Boguski, McCormick: "Proteins regulating Ras and its relatives." in: Nature, Vol. 366, Issue 6456, pp. 643-54, 1994 (PubMed).