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Details for Product No. ABIN650396

rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) antibody

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Antigen
Synonyms P160ROCK, ROCK-I, 1110055K06Rik, Rock-I, P160Rock, p160ROCK, LOC100229794, CG9774, DROK, DRhk, DRok, Dmel\\CG9774, Drok, ROCK, ROCK-1, ROCK1, ROK, ROKalpha/beta, Rhk, RhoK, Rock, Rok, dROK, dRok, drok
Reactivity
»Alternatives Human, Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Pubmed 3 references available
Catalog no. ABIN650396
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Immunogen A synthetic peptide corresponding to residues near C-terminus of human ROCK1 was used as an immunogen. This antibody recognizes both the cleaved C-terminus of ROCK-1 (30 k
Specificity A synthetic peptide corresponding to residues near C-terminus of human ROCK1 was used as an immunogen. This antibody recognizes both the cleaved C-terminus of ROCK-1 (30 kDa) and full length protein.
Alternative Name ROCK1
Background Rho-associated kinase I (ROCK1, ROK beta, p160ROCK) is a serine-threonine protein kinase and an effector of the small GTPase Rho. With N-terminus protein kinase domain and C-terminus Rho-binding domain/pleckstrin homology domain, ROCK1 can be activated by either RhoA or RhoB. In Rho specific ROCK1 activation, a Rho protein binds to the Rho-binding domain and induces a conformational change which opens the kinase domain for the phosphorylation of downstream effectors (1). Activated ROCK1 phosphorylates various signaling proteins, such as myosin light chain phosphatase, LIM kinases, and ezrin-radixin-moesin proteins. Caspase-3 also activates ROCK1, Caspase-3 cleaves ROCK1 at DETD1113/G sequence and removes its inhibitory c-terminal domain. This activation, independent of Rho activity, leads to apoptotic membrane blebbing (2). ROCK1 is involved in regulating actin cytoskeleton assembly, cell migration, centromere positing, smooth muscle contraction, and neurite outgrowth. Its involvement in tumor invasion, hypertension, and bronchial asthma makes ROCK1 an ideal target for drug development (3).
Synonyms: ROCK1, Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35
Molecular Weight 30/160 kDA
Gene ID 6093
UniProt Q13464
Research Area Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Chromatin
Application Notes The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:60 ICC: ~~ 1:250~500 IHC: ~~ 1:50~100
Comment

Background: Rho-associated kinase I (ROCK1, ROK beta, p160ROCK) is a serine-threonine protein kinase and an effector of the small GTPase Rho. With N-terminus protein kinase domain and C-terminus Rho-binding domain/pleckstrin homology domain, ROCK1 can be activated by either RhoA or RhoB. In Rho specific ROCK1 activation, a Rho protein binds to the Rho-binding domain and induces a conformational change which opens the kinase domain for the phosphorylation of downstream effectors (1). Activated ROCK1 phosphorylates various signaling proteins, such as myosin light chain phosphatase, LIM kinases, and ezrin-radixin-moesin proteins. Caspase-3 also activates ROCK1, Caspase-3 cleaves ROCK1 at DETD1113/G sequence and removes its inhibitory c-terminal domain. This activation, independent of Rho activity, leads to apoptotic membrane blebbing (2). ROCK1 is involved in regulating actin cytoskeleton assembly, cell migration, centromere positing, smooth muscle contraction, and neurite outgrowth. Its involvement in tumor invasion, hypertension, and bronchial asthma makes ROCK1 an ideal target for drug development (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment ROCK1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Coleman, Sahai, Yeo et al.: "Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I." in: Nature cell biology, Vol. 3, Issue 4, pp. 339-45, 2001 (PubMed).

Riento, Ridley: "Rocks: multifunctional kinases in cell behaviour." in: Nature reviews. Molecular cell biology, Vol. 4, Issue 6, pp. 446-56, 2003 (PubMed).

Dvorsky, Blumenstein, Vetter et al.: "Structural insights into the interaction of ROCKI with the switch regions of RhoA." in: The Journal of biological chemistry, Vol. 279, Issue 8, pp. 7098-104, 2004 (PubMed).

Alternatives for antigen "rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1)", type "Antibodies"
Hosts (65), (15)
Reactivities (78), (48), (47), (14), (12), (2), (2), (1), (1)
Applications (55), (27), (20), (15), (10), (6), (5), (3), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (12), (12), (8), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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