rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (C-Term) antibody

Details for Product No. ABIN650397
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Antigen
Synonyms LOC100229794, CG9774, DROK, DRhk, DRok, Dmel\\CG9774, Drok, ROCK, ROCK-1, ROCK1, ROK, ROKalpha/beta, Rhk, RhoK, Rock, Rok, dROK, dRok, drok, 1110055K06Rik, Rock-I, P160Rock, ROCK-I, P160ROCK, p160ROCK
Epitope
C-Term
(12), (12), (9), (8), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(82), (48), (48), (12), (12), (4), (1)
Host
Rabbit
(73), (13)
Clonality
Monoclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(66), (26), (20), (14), (10), (8), (6), (3)
Pubmed 3 references available
Quantity 100 μL
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Catalog No. ABIN650397
450.00 $
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Immunogen A synthetic peptide corresponding to residues near the C-terminus of human ROCK1 was used as an immunogen.
Specificity A synthetic peptide corresponding to residues near the C-terminus of human ROCK1 was used as an immunogen.
Alternative Name ROCK1
Background Rho-associated kinase I (ROCK1, ROK beta, p160ROCK) is a serine-threonine protein kinase and an effector of the small GTPase Rho. With N-terminus protein kinase domain and C-terminus Rho-binding domain/pleckstrin homology domain, ROCK1 can be activated by either RhoA or RhoB. In Rho specific ROCK1 activation, a Rho protein binds to the Rho-binding domain and induces a conformational change which opens the kinase domain for the phosphorylation of downstream effectors (1). Activated ROCK1 phosphorylates various signaling proteins, such as myosin light chain phosphatase, LIM kinases, and ezrin-radixin-moesin proteins. Caspase-3 also activates ROCK1, Caspase-3 cleaves ROCK1 at DETD1113/G sequence and removes its inhibitory c-terminal domain. This activation, independent of Rho activity, leads to apoptotic membrane blebbing (2). ROCK1 is involved in regulating actin cytoskeleton assembly, cell migration, centromere positing, smooth muscle contraction, and neurite outgrowth. Its involvement in tumor invasion, hypertension, and bronchial asthma makes ROCK1 an ideal target for drug development (3).
Synonyms: ROCK1, Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35
Molecular Weight 160 kDA
Gene ID 6093
UniProt Q13464
Research Area Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Chromatin
Application Notes IHC: = 1:50-100, WB: = 1:1000-10000
Comment

Background: Rho-associated kinase I (ROCK1, ROK beta, p160ROCK) is a serine-threonine protein kinase and an effector of the small GTPase Rho. With N-terminus protein kinase domain and C-terminus Rho-binding domain/pleckstrin homology domain, ROCK1 can be activated by either RhoA or RhoB. In Rho specific ROCK1 activation, a Rho protein binds to the Rho-binding domain and induces a conformational change which opens the kinase domain for the phosphorylation of downstream effectors (1). Activated ROCK1 phosphorylates various signaling proteins, such as myosin light chain phosphatase, LIM kinases, and ezrin-radixin-moesin proteins. Caspase-3 also activates ROCK1, Caspase-3 cleaves ROCK1 at DETD1113/G sequence and removes its inhibitory c-terminal domain. This activation, independent of Rho activity, leads to apoptotic membrane blebbing (2). ROCK1 is involved in regulating actin cytoskeleton assembly, cell migration, centromere positing, smooth muscle contraction, and neurite outgrowth. Its involvement in tumor invasion, hypertension, and bronchial asthma makes ROCK1 an ideal target for drug development (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment ROCK1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Coleman, Sahai, Yeo et al.: "Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I." in: Nature cell biology, Vol. 3, Issue 4, pp. 339-45, 2001 (PubMed).

Riento, Ridley: "Rocks: multifunctional kinases in cell behaviour." in: Nature reviews. Molecular cell biology, Vol. 4, Issue 6, pp. 446-56, 2003 (PubMed).

Dvorsky, Blumenstein, Vetter et al.: "Structural insights into the interaction of ROCKI with the switch regions of RhoA." in: The Journal of biological chemistry, Vol. 279, Issue 8, pp. 7098-104, 2004 (PubMed).

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