SATB Homeobox 1 (SATB1) antibody

Antigen: SATB Homeobox 1 (SATB1)

Synonyms: AW413156, 2610306G12Rik, SATB1, MGC165895

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650419

Additional Information

Alternative name: SATB1

Gene ID: 8578

UniProt: Q01826

Immunogen: A synthetic peptide corresponding to residues near the N-terminus in human SATB1 was used as an immunogen.

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the N-terminus in human SATB1 was used as an immunogen.

Molecular Weight: 100 kDA

Comments:

Background: Special AT-rich sequence-binding protein 1 (SATB1) is a global chromatin organizer and nuclear transcription factor which integrates higher-order chromatin architecture with gene regulation (1). SATB1 specifically binds to nuclear matrix/scaffold-associating DNAs (MARs/SARs), which is made of AT-rich DNA sequences with one strand consisting of ATC sequences. Studies have shown that in cooperation with promyelocytic leukemia protein (PML), SATB1 and PML function as a regulatory complex which controls transcription through restructuring dynamic chromatin-loop architecture. SATB1 interacts with PML to organize the MHC class-I locus into distinct higher-order chromatin loops by anchoring MARs to nuclear matrix at fixed distances (2). As a key player in the immune system, SATB1 has been linked to regulating gene expression during the differentiation and activation of T cells. In cancer, SATB1 has been shown to reprogram chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:10~100 ICC: ~~ 1:50~100 IHC: ~~ 1:50~100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: SATB1 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Kumar, Bischof, Purbey et al.: "Functional interaction between PML and SATB1 regulates chromatin-loop architecture and transcription of the MHC class I locus." in: Nature cell biology, Vol. 9, Issue 1, pp. 45-56, 2007 (PubMed).

Galande, Purbey, Notani et al.: "The third dimension of gene regulation: organization of dynamic chromatin loopscape by SATB1." in: Current opinion in genetics & development, Vol. 17, Issue 5, pp. 408-14, 2007 (PubMed).

Han, Russo, Kohwi et al.: "SATB1 reprogrammes gene expression to promote breast tumour growth and metastasis." in: Nature, Vol. 452, Issue 7184, pp. 187-93, 2008 (PubMed).