Pituitary Tumor-Transforming 1 (PTTG1) antibody

Details for Product No. ABIN650423
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Antigen
Synonyms EAP1, HPTTG, PTTG, TUTR1, AW555095, C87862, Pttg, Pttg3, Securin, PTTG1, securin
Reactivity
Human
(77), (31), (25), (12), (1)
Host
Rabbit
(54), (20), (3)
Clonality
Monoclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(47), (38), (21), (20), (18), (10), (8), (3), (2), (1)
Pubmed 2 references available
Catalog no. ABIN650423
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Immunogen A synthetic peptide corresponding to residues near the N-terminus of human securin was used as an immunogen.
Specificity A synthetic peptide corresponding to residues near the N-terminus of human securin was used as an immunogen.
Alternative Name Securin
Background Securin is a regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Securin acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Securin negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the securin when it is overexpressed (1). It may also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation (2).
Synonyms: PTTG1, EAP1, PTTG, TUTR1, Securin, Esp1-associated protein
Molecular Weight 28 kDA
Gene ID 9232
UniProt O95997
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Chromatin, Cell Structure
Application Notes IHC: = 1:100-250, WB: = 1:100000-500000
Comment

Background: Securin is a regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Securin acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Securin negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the securin when it is overexpressed (1). It may also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation (2).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Securin Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Romero, Multon, Ramos-Morales et al.: "Human securin, hPTTG, is associated with Ku heterodimer, the regulatory subunit of the DNA-dependent protein kinase." in: Nucleic acids research, Vol. 29, Issue 6, pp. 1300-7, 2001 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).

Hosts (54), (20), (3)
Reactivities (77), (31), (25), (12), (1)
Applications (47), (38), (21), (20), (18), (10), (8), (3), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (12), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1)
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