Pituitary Tumor-Transforming 1 (PTTG1) antibody

Antigen: Pituitary Tumor-Transforming 1 (PTTG1)

Synonyms: EAP1, PTTG, HPTTG, TUTR1, MGC126883, MGC138276, PTTG1, MGC127932, MGC134134

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650423

Additional Information

Alternative name: Securin

Gene ID: 9232

UniProt: O95997

Immunogen: A synthetic peptide corresponding to residues near the N-terminus of human securin was used as an immunogen.

Description: Other names: PTTG1, EAP1, PTTG, TUTR1, Securin, Esp1-associated protein

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the N-terminus of human securin was used as an immunogen.

Molecular Weight: 28 kDA

Comments:

Background: Securin is a regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Securin acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Securin negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the securin when it is overexpressed (1). It may also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation (2).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:100000~500000 IP: ~~ 1:30 FC: ~~ 1:50 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Securin Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Romero, Multon, Ramos-Morales et al.: "Human securin, hPTTG, is associated with Ku heterodimer, the regulatory subunit of the DNA-dependent protein kinase." in: Nucleic acids research, Vol. 29, Issue 6, pp. 1300-7, 2001 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).