SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) (pTyr239), (pTyr240) antibody

Details for Product No. ABIN650428
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Antigen
Synonyms SHC, p66shc, SHCA, P66shc, shc, SHC1
Epitope
pTyr239, pTyr240
(34), (32), (14), (14), (13), (13), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Reactivity
Human
(181), (157), (134), (29), (25), (24), (1), (1), (1)
Host
Rabbit
(172), (14)
Clonality
Monoclonal
Conjugate
Un-conjugated
(7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application
Western Blotting (WB)
(124), (60), (52), (51), (37), (32), (15), (13), (4), (3), (2), (1)
Pubmed 3 references available
Catalog no. ABIN650428
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
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Immunogen A synthetic peptide corresponding to residues surrounding Tyrosine 239/240 of human SHC1 was used as an immunogen. The antibody only detects SHC1 phosphorylated at Tyrosine 239/240.
Specificity A synthetic peptide corresponding to residues surrounding Tyrosine 239/240 of human SHC1 was used as an immunogen. The antibody only detects SHC1 phosphorylated at Tyrosine 239/240.
Alternative Name SHC1
Background The human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p66). All three SHC isoforms are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are expressed in every cell type while p66 expression varies from cell type to cell type (1-3). Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p66 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p66 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and life span (4-5).
Synonyms: SHC1, SHC, SHCA, SHC-transforming protein 1, Src homology 2 domain-containing-transforming protein C1
Molecular Weight 42/52/67 kDA
Gene ID 6464
UniProt P29353
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure
Application Notes The suggested dilution is: WB: = 1:500-1000
Comment

Background: The human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p66). All three SHC isoforms are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are expressed in every cell type while p66 expression varies from cell type to cell type (1-3). Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p66 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p66 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and life span (4-5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment SHC1 Antibody Phospho (pY239/240) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Pelicci, Lanfrancone, Grignani et al.: "A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction." in: Cell, Vol. 70, Issue 1, pp. 93-104, 1992 (PubMed).

Migliaccio, Giorgio, Mele et al.: "The p66shc adaptor protein controls oxidative stress response and life span in mammals." in: Nature, Vol. 402, Issue 6759, pp. 309-13, 1999 (PubMed).

Ventura, Luzi, Pacini et al.: "The p66Shc longevity gene is silenced through epigenetic modifications of an alternative promoter." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22370-6, 2002 (PubMed).

Hosts (172), (14)
Reactivities (181), (157), (134), (29), (25), (24), (1), (1), (1)
Applications (124), (60), (52), (51), (37), (32), (15), (13), (4), (3), (2), (1)
Conjugates (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Epitopes (34), (32), (14), (14), (13), (13), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
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