Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D) (pTyr1020) antibody

Details for Product No. ABIN650431
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Antigen
Synonyms INPP5D, 256.t00004, 13.t00027, ship, ship1, AI323613, SHIP, SHIP-1, SHIP1, s-SHIP, SIP-145, hp51CN
Epitope
pTyr1020
(20), (13), (9), (8), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(86), (56), (53), (24), (12), (12)
Host
Rabbit
(80), (9)
Clonality
Monoclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(59), (32), (20), (18), (4), (2), (1)
Pubmed 3 references available
Quantity 100 μL
Options
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Catalog No. ABIN650431
507.14 $
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Immunogen A synthetic peptide corresponding to residues surrounding Tyrosine 1020 of human SHIP was used as an immunogen.
Specificity A synthetic peptide corresponding to residues surrounding Tyrosine 1020 of human SHIP was used as an immunogen.
Alternative Name SHIP
Background SH2-containing inositol phosphatase 1 (SHIP) is a 145 kDa hemopoietic-specific phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate. SHIP has been shown to bind directly a phosphorylated motif, immunoreceptor tyrosine-based inhibition motif (ITIM), present in the cytoplasmic domain of FcgammaRIIB1 (1). Also, results demonstrate that FcgammaRIIB use SHIP to inhibit pathways shared by receptor tyrosine kinases and immunoreceptors to trigger cell proliferation and cell activation, respectively (2). Phosphorylation on tyrosine 917 and tyrosine 1020 sites in SHIP have been shown to control migration by binding to DOK1, a regulating protein linked to the regulation of migration (3).
Molecular Weight 145 kDA
Gene ID 1786
UniProt O00145
Research Area Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Metabolism
Application Notes The suggested dilution is: WB: = 1:2000
Comment

Background: SH2-containing inositol phosphatase 1 (SHIP) is a 145 kDa hemopoietic-specific phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate. SHIP has been shown to bind directly a phosphorylated motif, immunoreceptor tyrosine-based inhibition motif (ITIM), present in the cytoplasmic domain of FcgammaRIIB1 (1). Also, results demonstrate that FcgammaRIIB use SHIP to inhibit pathways shared by receptor tyrosine kinases and immunoreceptors to trigger cell proliferation and cell activation, respectively (2). Phosphorylation on tyrosine 917 and tyrosine 1020 sites in SHIP have been shown to control migration by binding to DOK1, a regulating protein linked to the regulation of migration (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment SHIP Antibody Phospho (pY1020) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Tridandapani, Kelley, Pradhan et al.: "Recruitment and phosphorylation of SH2-containing inositol phosphatase and Shc to the B-cell Fc gamma immunoreceptor tyrosine-based inhibition motif peptide motif." in: Molecular and cellular biology, Vol. 17, Issue 8, pp. 4305-11, 1997 (PubMed).

Sattler, Verma, Pride et al.: "SHIP1, an SH2 domain containing polyinositol-5-phosphatase, regulates migration through two critical tyrosine residues and forms a novel signaling complex with DOK1 and CRKL." in: The Journal of biological chemistry, Vol. 276, Issue 4, pp. 2451-8, 2001 (PubMed).

Malbec, Schmitt, Bruhns et al.: "Src homology 2 domain-containing inositol 5-phosphatase 1 mediates cell cycle arrest by FcgammaRIIB." in: The Journal of biological chemistry, Vol. 276, Issue 32, pp. 30381-91, 2001 (PubMed).

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