Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D) (pTyr1020) antibody

Antigen: Inositol Polyphosphate-5-Phosphatase, 145kDa (INPP5D)

Synonyms: SHIP, SHIP1, hp51CN, SIP-145, MGC104855, MGC142140, MGC142142, INPP5D, MGC157261, inpp5d, ship, ship1, MGC68993

Binding Site: pTyr1020

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650431

Additional Information

Alternative name: SHIP

Gene ID: 1786

UniProt: O00145

Immunogen: A synthetic peptide corresponding to residues surrounding Tyrosine 1020 of human SHIP was used as an immunogen.

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues surrounding Tyrosine 1020 of human SHIP was used as an immunogen.

Molecular Weight: 145 kDA

Comments:

Background: SH2-containing inositol phosphatase 1 (SHIP) is a 145 kDa hemopoietic-specific phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate. SHIP has been shown to bind directly a phosphorylated motif, immunoreceptor tyrosine-based inhibition motif (ITIM), present in the cytoplasmic domain of FcgammaRIIB1 (1). Also, results demonstrate that FcgammaRIIB use SHIP to inhibit pathways shared by receptor tyrosine kinases and immunoreceptors to trigger cell proliferation and cell activation, respectively (2). Phosphorylation on tyrosine 917 and tyrosine 1020 sites in SHIP have been shown to control migration by binding to DOK1, a regulating protein linked to the regulation of migration (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:2000 IP: ~~ 1:20 ICC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: SHIP Antibody Phospho (pY1020) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Metabolism

Restrictions: For Research Use only

Publications

Product: Tridandapani, Kelley, Pradhan et al.: "Recruitment and phosphorylation of SH2-containing inositol phosphatase and Shc to the B-cell Fc gamma immunoreceptor tyrosine-based inhibition motif peptide motif." in: Molecular and cellular biology, Vol. 17, Issue 8, pp. 4305-11, 1997 (PubMed).

Sattler, Verma, Pride et al.: "SHIP1, an SH2 domain containing polyinositol-5-phosphatase, regulates migration through two critical tyrosine residues and forms a novel signaling complex with DOK1 and CRKL." in: The Journal of biological chemistry, Vol. 276, Issue 4, pp. 2451-8, 2001 (PubMed).

Malbec, Schmitt, Bruhns et al.: "Src homology 2 domain-containing inositol 5-phosphatase 1 mediates cell cycle arrest by FcgammaRIIB." in: The Journal of biological chemistry, Vol. 276, Issue 32, pp. 30381-91, 2001 (PubMed).