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Second Mitochondria-Derived Activator of Caspase (DIABLO) antibody

Antigen

Second Mitochondria-Derived Activator of Caspase (DIABLO)
Synonyms SMAC, SMAC3, DIABLO-S, FLJ10537, FLJ25049, smac, smac3, diablo-s, XSmac, diablo
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)
1 reference available
Certificates ISO 9001:2008
Catalog no. ABIN650447
Quantity 100ul
Price 450.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Smac/Diablo
Gene ID 56616
UniProt Q9NR28
Immunogen A synthetic peptide corresponding to C-terminal residues human Smac/DIABLO was used as immunogen.
Description Other names: DIABLO, SMAC, Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to C-terminal residues human Smac/DIABLO was used as immunogen.
Molecular Weight 21/27 kDA
Comments

Background: Smac/Diablo (Second Mitochondria-derived Activator of Caspases/Direct IAP Binding Protein with Low PI) is localized to the mitochondria and involved in activating programmed cell death, or apoptosis (1). In response to apoptotic stimuli, Smac/Diablo is released from the mitochondria and binds anti-apoptotic IAP proteins, supressing their inhibitory activity and promoting caspase activation (1,2). This interaction involves primarily the amino-terminal residues of Smac/Diablo with the BIR3 region of the IAP protein (3,4).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:10 ICC: ~~ 1:10 IHC: ~~ 1:10
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Smac/Diablo Antibody can be stored at -20°C for up to 12 months from time of receipt.
Research Area Apoptosis/Necrosis, Cell Signaling, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Du, Fang, Li et al.: "Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition." in: Cell, Vol. 102, Issue 1, pp. 33-42, 2000 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Second Mitochondria-Derived Activator of Caspase (DIABLO)", type "Antibodies"
Hosts Rabbit (78), Mouse (11), Rat (4)
Reactivities Human (82), Mouse (Murine) (42), Rat (Rattus) (36), Cow (Bovine) (20), Dog (Canine) (20), Chicken (19), Pig (Porcine) (19), Horse (Equine) (18), Gerbil (2), Cat (Feline) (1), Monkey (1)
Applications Western Blotting (WB) (74), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (32), ELISA (26), Immunofluorescence (IF) (20), Immunoprecipitation (IP) (17), Immunohistochemistry (IHC) (15), Immunocytochemistry (ICC) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Flow Cytometry (FACS) (3), Immunohistochemistry (Fixed) (IHC (fx)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes C-Term (16), AA 199-212 (4), AA 225-239 (4), AA 222-237 (3), AA 56-239 (2), AA 1-55 (1), AA 226-239 (1), AA 56-239,His6 (1), AA 56-73 (1)