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SMAD, Mothers Against DPP Homolog 2 (SMAD2) (MH2 domain) antibody

Details for Product No. ABIN650449
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Antigen
Synonyms jv18, Xmad2, madh2, madr2, XSmad2, hmad-2, hsmad2, jv18-1, smad-2, fj43c06, wu:fj43c06, JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2, 7120426M23Rik, Madh2, Madr2, Smad-2, mMad2
Epitope
MH2 domain
(40), (36), (33), (22), (21), (18), (14), (13), (13), (12), (8), (7), (7), (6), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(334), (191), (185), (79), (75), (65), (13), (3), (1), (1), (1), (1)
Host
Rabbit
(294), (35), (12)
Clonality
Monoclonal
Conjugate
Un-conjugated
(12), (10), (10), (6), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(261), (141), (64), (60), (60), (48), (35), (18), (10), (6), (5), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 µL
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Catalog No. ABIN650449
450.00 $
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Immunogen A synthetic peptide corresponding to residues near the MH2 domain of human Smad2 was used as immunogen.
Specificity A synthetic peptide corresponding to residues near the MH2 domain of human Smad2 was used as immunogen.
Alternative Name Smad2
Background Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided in 3 subgroups based on their structure and functions, pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).
Synonyms: SMAD2, MADH2, MADR2, Mothers against decapentaplegic homolog 2, SMAD 2,hSMAD2,JV18-1
Molecular Weight 58 kDA
Gene ID 4087
UniProt Q15796
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure
Application Notes IHC: = 1:50, WB: = 1:10000-50000
Comment

Background: Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided in 3 subgroups based on their structure and functions, pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Smad2 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Eppert, Scherer, Ozcelik et al.: "MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma." in: Cell, Vol. 86, Issue 4, pp. 543-52, 1996 (PubMed).

Validation Images
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