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Details for Product No. ABIN650450

SMAD, Mothers Against DPP Homolog 2 (SMAD2) antibody

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Antigen
Synonyms MADH2, madh2, fj43c06, wu:fj43c06, XSmad2, smad-2, JV18, JV18-1, MADR2, hMAD-2, hSMAD2, 7120426M23Rik, Madh2, Madr2, Smad-2, mMad2, jv18, Xmad2, madr2, hmad-2, hsmad2, jv18-1
Reactivity
»Alternatives Human, Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
Pubmed 2 references available
Catalog no. ABIN650450
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen A synthetic peptide corresponding to residues surrounding the MH1 domain of human Smad2 was used as immunogen.
Specificity A synthetic peptide corresponding to residues surrounding the MH1 domain of human Smad2 was used as immunogen.
Alternative Name Smad2
Background Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions, pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).
Synonyms: SMAD2, MADH2, MADR2, Mothers against decapentaplegic homolog 2, SMAD 2,hSMAD2,JV18-1
Molecular Weight 58 kDA
Gene ID 4087
UniProt Q15796
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:1000 IP: ~~ 1:50 FC: ~~ 1:30 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250
Comment

Background: Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions, pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Smad2 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Eppert, Scherer, Ozcelik et al.: "MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma." in: Cell, Vol. 86, Issue 4, pp. 543-52, 1996 (PubMed).

Chen, Rubock, Whitman: "A transcriptional partner for MAD proteins in TGF-beta signalling." in: Nature, Vol. 383, Issue 6602, pp. 691-6, 1996 (PubMed).

Alternatives for antigen "SMAD, Mothers Against DPP Homolog 2 (SMAD2)", type "Antibodies"
Hosts (253), (31), (13)
Reactivities (291), (193), (188), (82), (77), (67), (13), (3), (3), (3), (2), (1), (1), (1), (1)
Applications (230), (117), (60), (54), (53), (43), (16), (12), (8), (6), (4), (3), (2), (1)
Conjugates (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (4)
Epitopes (34), (32), (27), (19), (18), (13), (13), (12), (8), (6), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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