Ras Homolog Gene Family, Member Q (RHOQ) antibody

Antigen: Ras Homolog Gene Family, Member Q (RHOQ)

Synonyms: ARHQ, TC10, TC10A, RASL7A, Tc10, Arhq, zgc:55997, wu:fi15a09, RHOQ, CHTC10

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN650521

Additional Information

Alternative name: TC10

Gene ID: 23433

UniProt: P17081

Immunogen: A synthetic peptide corresponding to the third GTP unit of human TC10 was used as immunogen.

Description: Other names: RHOQ, ARHQ, RASL7A, TC10, Rho-related GTP-binding protein RhoQ, Ras-related GTP-binding protein TC10,Ras-like protein family member 7A

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to the third GTP unit of human TC10 was used as immunogen.

Molecular Weight: 25 kDA

Comments:

Background: TC10 is a member of the Rho family of GTP-binding proteins and is closely related to Cdc42 and Rac 1. Cycling between inactive and active GTP-bound state, active TC10 can directly interact with Cdc42 kinase, p21-activated protein kinases as well as other kinases (1). Constitutive expression of active TC10 mutants (TC10/Q75L) in fibroblast decrease actin stress fiber and formation of plasma membrane microspikes, while wild type TC10 has no effect on fibroblast cell morphology (2). Mostly expressed in adipose and muscle tissues, TC10 has been found to be activated by C3G (from the insulin pathway) in adipocytes (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 ICC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: TC10 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Protein Modifications, Cell Structure, Chromatin

Restrictions: For Research Use only

Publications

Product: Murphy, Solski, Jillian et al.: "Cellular functions of TC10, a Rho family GTPase: regulation of morphology, signal transduction and cell growth." in: Oncogene, Vol. 18, Issue 26, pp. 3831-45, 1999 (PubMed).

Baumann, Ribon, Kanzaki et al.: "CAP defines a second signalling pathway required for insulin-stimulated glucose transport." in: Nature, Vol. 407, Issue 6801, pp. 202-7, 2000 (PubMed).