Transcription Factor 3 (TCF3) antibody

Antigen: Transcription Factor 3 (TCF3)

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN650522

Additional Information

Alternative name: TCF-3

Gene ID: 83439

UniProt: Q9HCS4

Immunogen: A synthetic peptide corresponding to residues in human TCF-3 was used as an immunogen.

Description: Other names: TCF7L1, TCF3, Transcription factor 7-like 1, HMG box transcription factor 3

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human TCF-3 was used as an immunogen.

Molecular Weight: 68 kDA

Comments:

Background: T-cell factor 3 (transcription factor 7-like 1, TCF-3) is a component of the Wnt signaling pathway, which functions in CNS patterning, proliferation and neurogenesis, and a dominant downstream effector in embryonic stem cells (ESCs). TCF-3 binds to and represses the Oct4 promoter, and plays an important role in modulating the appropriate level of gene transcription in ESCs and during embryonic development (1, 2). TCF-3 is necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis. It down-regulates NQO1, leading to increased mitomycin c resistance. It is detected in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium (2).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~5000 FC: ~~ 1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: TCF-3 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Translation Factors, Cell Signaling, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: "The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).

Tam, Lim, Han et al.: "T-cell factor 3 regulates embryonic stem cell pluripotency and self-renewal by the transcriptional control of multiple lineage pathways." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 8, pp. 2019-31, 2008 (PubMed).