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T-Complex 1 (TCP1) (N-Term) antibody

Details for Product No. ABIN650524
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Antigen
Synonyms
BEST:GH05123, CCT1, CG5374, Dmel\\CG5374, T-cpl, TCP-1alpha, TCPA_DROME, Tcp-1, Tcp1, Tcp1-alpha, gh05123, Cct1-1P, CCT-alpha, CCTa, D6S230E, TCP-1-alpha, CCT, TRiC, CCTalpha, AI528772, Cct1, Ccta, TR ... show more
BEST:GH05123, CCT1, CG5374, Dmel\\CG5374, T-cpl, TCP-1alpha, TCPA_DROME, Tcp-1, Tcp1, Tcp1-alpha, gh05123, Cct1-1P, CCT-alpha, CCTa, D6S230E, TCP-1-alpha, CCT, TRiC, CCTalpha, AI528772, Cct1, Ccta, TRic, Tp63, c-cpn, p63 show less
Epitope
N-Term
(55), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(63), (38), (18), (16), (15), (14), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(51), (25), (8), (4)
Clonality
Monoclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(77), (53), (28), (27), (25), (21), (12), (10), (9), (1)
Pubmed 5 references available
Quantity 100 µL
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Catalog No. ABIN650524
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Immunogen A synthetic peptide corresponding to residues near the N-terminus in human TCP1 Alpha was used as an immunogen.
Specificity A synthetic peptide corresponding to residues near the N-terminus in human TCP1 Alpha was used as an immunogen.
Alternative Name TCP1 alpha
Background T-complex polypeptide 1 subunit alpha (TCP1 alpha) is a cytosolic molecular chaperone that belongs to the chaperonin-containing TCP1 (CCT) complex (1). The CCT complex is composed of eight subunits that assist in the folding of actin, tubulin, and other cytosolic proteins (1). The sequence differences among the CCT subunits are located mainly in their apical domains, suggesting some degree of specificity towards substrate binding (2). TCP1 alpha plays a role in the folding of newly translated proteins in the cytosol (3). Major cytoskeletal proteins such as actin and tubulin, depend on CCT to maintain the integrity of the microfilament and microtubule-based cytoskeletal systems (4). Overexpression of the TCP1 alpha in neurons may reduce the formation of pathogenic conformations of the hungtingtin protein associated with Huntington's disease (5).
Molecular Weight 60 kDA
Gene ID 10576
UniProt P17987
Research Area Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-10000 ,IHC: = 1: 100-250
Comment

Background: T-complex polypeptide 1 subunit alpha (TCP1 alpha) is a cytosolic molecular chaperone that belongs to the chaperonin-containing TCP1 (CCT) complex (1). The CCT complex is composed of eight subunits that assist in the folding of actin, tubulin, and other cytosolic proteins (1). The sequence differences among the CCT subunits are located mainly in their apical domains, suggesting some degree of specificity towards substrate binding (2). TCP1 alpha plays a role in the folding of newly translated proteins in the cytosol (3). Major cytoskeletal proteins such as actin and tubulin, depend on CCT to maintain the integrity of the microfilament and microtubule-based cytoskeletal systems (4). Overexpression of the TCP1 alpha in neurons may reduce the formation of pathogenic conformations of the hungtingtin protein associated with Huntington's disease (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment TCP1 alpha Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Llorca, Martín-Benito, Grantham et al.: "The 'sequential allosteric ring' mechanism in the eukaryotic chaperonin-assisted folding of actin and tubulin." in: The EMBO journal, Vol. 20, Issue 15, pp. 4065-75, 2001 (PubMed).

Yokota, Yanagi, Yura et al.: "Cytosolic chaperonin-containing t-complex polypeptide 1 changes the content of a particular subunit species concomitant with substrate binding and folding activities during the cell cycle." in: European journal of biochemistry / FEBS, Vol. 268, Issue 17, pp. 4664-73, 2001 (PubMed).

Tam, Geller, Spiess et al.: "The chaperonin TRiC controls polyglutamine aggregation and toxicity through subunit-specific interactions." in: Nature cell biology, Vol. 8, Issue 10, pp. 1155-62, 2006 (PubMed).

Brackley, Grantham: "Activities of the chaperonin containing TCP-1 (CCT): implications for cell cycle progression and cytoskeletal organisation." in: Cell stress & chaperones, Vol. 14, Issue 1, pp. 23-31, 2008 (PubMed).

Bichet: "V2R mutations and nephrogenic diabetes insipidus." in: Progress in molecular biology and translational science, Vol. 89, pp. 15-29, 2010 (PubMed).

Validation Images
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