T-Complex 1 (TCP1) antibody

Antigen: T-Complex 1 (TCP1)

Synonyms: CCT1, CCTa, D6S230E, CCT-alpha, TCP-1-alpha, Cct1-1P

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650524

Additional Information

Alternative name: TCP1 alpha

Gene ID: 10576

UniProt: P17987

Immunogen: A synthetic peptide corresponding to residues near the N-terminus in human TCP1 Alpha was used as an immunogen.

Characteristics: Rabbit Monoclonal IgG

Specificity: A synthetic peptide corresponding to residues near the N-terminus in human TCP1 Alpha was used as an immunogen.

Molecular Weight: 60 kDA

Comments:

Background: T-complex polypeptide 1 subunit alpha (TCP1 alpha) is a cytosolic molecular chaperone that belongs to the chaperonin-containing TCP1 (CCT) complex (1). The CCT complex is composed of eight subunits that assist in the folding of actin, tubulin, and other cytosolic proteins (1). The sequence differences among the CCT subunits are located mainly in their apical domains, suggesting some degree of specificity towards substrate binding (2). TCP1 alpha plays a role in the folding of newly translated proteins in the cytosol (3). Major cytoskeletal proteins such as actin and tubulin, depend on CCT to maintain the integrity of the microfilament and microtubule-based cytoskeletal systems (4). Overexpression of the TCP1 alpha in neurons may reduce the formation of pathogenic conformations of the hungtingtin protein associated with Huntington's disease (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:10~100 ICC: ~~ 1: 250~500 IHC: ~~ 1: 100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: TCP1 alpha Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Llorca, Martín-Benito, Grantham et al.: "The 'sequential allosteric ring' mechanism in the eukaryotic chaperonin-assisted folding of actin and tubulin." in: The EMBO journal, Vol. 20, Issue 15, pp. 4065-75, 2001 (PubMed).

Yokota, Yanagi, Yura et al.: "Cytosolic chaperonin-containing t-complex polypeptide 1 changes the content of a particular subunit species concomitant with substrate binding and folding activities during the cell cycle." in: European journal of biochemistry / FEBS, Vol. 268, Issue 17, pp. 4664-73, 2001 (PubMed).

Tam, Geller, Spiess et al.: "The chaperonin TRiC controls polyglutamine aggregation and toxicity through subunit-specific interactions." in: Nature cell biology, Vol. 8, Issue 10, pp. 1155-62, 2006 (PubMed).

Brackley, Grantham: "Activities of the chaperonin containing TCP-1 (CCT): implications for cell cycle progression and cytoskeletal organisation." in: Cell stress & chaperones, Vol. 14, Issue 1, pp. 23-31, 2008 (PubMed).

Bichet: "V2R mutations and nephrogenic diabetes insipidus." in: Progress in molecular biology and translational science, Vol. 89, pp. 15-29, 2010 (PubMed).