Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2) antibody

Antigen: Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2)

Synonyms: CCTB, 99D8.1, PRO1633, CCT-beta, MGC142074, MGC142076, TCP-1-beta, fa26a01, fc58a11, CHUNP6883, wu:fa26a01, wu:fc58a11, cct2, MGC89448, CCT2, MGC128852, Cctb, MGC94480

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650525

Additional Information

Alternative name: TCP1 beta

Gene ID: 3718

UniProt: P78371

Immunogen: A synthetic peptide corresponding to residues on the C-terminus in human TCP1 Beta was used as an immunogen.

Characteristics: Rabbit Monoclonal IgG

Specificity: A synthetic peptide corresponding to residues on the C-terminus in human TCP1 Beta was used as an immunogen.

Molecular Weight: 50 kDA

Comments:

Background: T-complex polypeptide 1 subunit beta (TCP1 beta) is a cytosolic molecular chaperone that belongs to the chaperonin-containing TCP1 (CCT) complex (1). The CCT complex is composed of eight subunits that assist in the folding of actin, tubulin, and other cytosolic proteins (1). The sequence differences among the CCT subunits are located mainly in their apical domains, suggesting some degree of specificity towards substrate binding (2). TCP1 beta plays a role in the folding of newly translated proteins in the cytosol (3). Major cytoskeletal proteins such as actin and tubulin, depend on CCT to maintain the integrity of the microfilament and microtubule-based cytoskeletal systems (4). The beta-tubulin/TCP1 beta complex may serve as an effective chemotherapeutic target for treating tumors overexpressing TCP1 beta (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:2000 IP: ~~ 1:50 FC: ~~ 1:20 ICC: ~~ 1: 100~250 IHC: ~~ 1: 100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: TCP1 beta Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Llorca, Martín-Benito, Grantham et al.: "The 'sequential allosteric ring' mechanism in the eukaryotic chaperonin-assisted folding of actin and tubulin." in: The EMBO journal, Vol. 20, Issue 15, pp. 4065-75, 2001 (PubMed).

Yokota, Yanagi, Yura et al.: "Cytosolic chaperonin-containing t-complex polypeptide 1 changes the content of a particular subunit species concomitant with substrate binding and folding activities during the cell cycle." in: European journal of biochemistry / FEBS, Vol. 268, Issue 17, pp. 4664-73, 2001 (PubMed).

Brackley, Grantham: "Activities of the chaperonin containing TCP-1 (CCT): implications for cell cycle progression and cytoskeletal organisation." in: Cell stress & chaperones, Vol. 14, Issue 1, pp. 23-31, 2008 (PubMed).

Lin, Tsai, Liu et al.: "Intracellular beta-tubulin/chaperonin containing TCP1-beta complex serves as a novel chemotherapeutic target against drug-resistant tumors." in: Cancer research, Vol. 69, Issue 17, pp. 6879-88, 2009 (PubMed).

Bichet: "V2R mutations and nephrogenic diabetes insipidus." in: Progress in molecular biology and translational science, Vol. 89, pp. 15-29, 2010 (PubMed).