|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Chaperonin Containing TCP1, Subunit 2 (Beta) (CCT2) antibody
|Synonyms||CCTB, 99D8.1, PRO1633, CCT-beta, MGC142074, MGC142076, TCP-1-beta, fa26a01, fc58a11, CHUNP6883, wu:fa26a01, wu:fc58a11, cct2, MGC89448, CCT2, MGC128852, Cctb, MGC94480|
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||TCP1 beta|
|Immunogen||A synthetic peptide corresponding to residues on the C-terminus in human TCP1 Beta was used as an immunogen.|
|Characteristics||Rabbit Monoclonal IgG|
|Specificity||A synthetic peptide corresponding to residues on the C-terminus in human TCP1 Beta was used as an immunogen.|
|Molecular Weight||50 kDA|
Background: T-complex polypeptide 1 subunit beta (TCP1 beta) is a cytosolic molecular chaperone that belongs to the chaperonin-containing TCP1 (CCT) complex (1). The CCT complex is composed of eight subunits that assist in the folding of actin, tubulin, and other cytosolic proteins (1). The sequence differences among the CCT subunits are located mainly in their apical domains, suggesting some degree of specificity towards substrate binding (2). TCP1 beta plays a role in the folding of newly translated proteins in the cytosol (3). Major cytoskeletal proteins such as actin and tubulin, depend on CCT to maintain the integrity of the microfilament and microtubule-based cytoskeletal systems (4). The beta-tubulin/TCP1 beta complex may serve as an effective chemotherapeutic target for treating tumors overexpressing TCP1 beta (5).
|Application Notes||The suggested dilution is: WB: ~~ 1:2000 IP: ~~ 1:50 FC: ~~ 1:20 ICC: ~~ 1: 100~250 IHC: ~~ 1: 100~250|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||TCP1 beta Antibody can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Signaling, Protein Modifications, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Llorca, Martín-Benito, Grantham et al.: "The 'sequential allosteric ring' mechanism in the eukaryotic chaperonin-assisted folding of actin and tubulin." in: The EMBO journal, Vol. 20, Issue 15, pp. 4065-75, 2001 (PubMed).
Yokota, Yanagi, Yura et al.: "Cytosolic chaperonin-containing t-complex polypeptide 1 changes the content of a particular subunit species concomitant with substrate binding and folding activities during the cell cycle." in: European journal of biochemistry / FEBS, Vol. 268, Issue 17, pp. 4664-73, 2001 (PubMed).
Brackley, Grantham: "Activities of the chaperonin containing TCP-1 (CCT): implications for cell cycle progression and cytoskeletal organisation." in: Cell stress & chaperones, Vol. 14, Issue 1, pp. 23-31, 2008 (PubMed).
Lin, Tsai, Liu et al.: "Intracellular beta-tubulin/chaperonin containing TCP1-beta complex serves as a novel chemotherapeutic target against drug-resistant tumors." in: Cancer research, Vol. 69, Issue 17, pp. 6879-88, 2009 (PubMed).
Bichet: "V2R mutations and nephrogenic diabetes insipidus." in: Progress in molecular biology and translational science, Vol. 89, pp. 15-29, 2010 (PubMed).
|Hosts||Rabbit (26), Mouse (12), Rat (6)|
|Reactivities||Human (38), Mouse (Murine) (22), Cow (Bovine) (20), Rat (Rattus) (20), Pig (Porcine) (18), Rabbit (18), Zebrafish (Danio rerio) (18), Monkey (2)|
|Applications||Western Blotting (WB) (28), Immunofluorescence (IF) (18), ELISA (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (IHC) (3), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)|
|Epitopes||N-Term (4), C-Term (2)|