Thymidine Kinase 1, Soluble (TK1) antibody

Antigen: Thymidine Kinase 1, Soluble (TK1)

Synonyms: TK2, Tk-1, Tk1a, Tk1b, D530002A18Rik, fi19b06, zgc:55362, wu:fi19b06, TK, TK1, tk2, MGC148510, Tk

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN650533

Additional Information

Alternative name: Thymidine Kinase 1

Gene ID: 7083

UniProt: P04183

Immunogen: A synthetic peptide corresponding to residues in human Thymidine Kinase 1 was used as an immunogen.

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human Thymidine Kinase 1 was used as an immunogen.

Molecular Weight: 24 kDA

Comments:

Background: Cytosolic thymidine kinase 1, TK1, is a well known cell-cycle-regulated enzyme of importance in nucleotide metabolism as well as an activator of antiviral and anticancer drugs (1). TK1 catalyzes the ATP-dependent phosphorylation of thymidine to thymidine 5'-monophosphate, which is subsequently converted to thymidine 5'-triphosphate and utilized for DNA synthesis. In terminally differentiating cells, such as cardiac muscle cells, the cessation of DNA synthesis and withdrawal from the cell cycle are accompanied by the loss of TK1 activity (2). TK1 is a key enzyme of the salvage pathway for pyrimidine deoxyribonucleotide synthesis in a variety of organisms (3). Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle, it is very low in resting cells (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~5000 IP: ~~ 1:10 FC: ~~ 1:200 ICC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Thymidine Kinase 1 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Cell Signaling, Protein Modifications, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Publications

Product: Sherley, Kelly: "Human cytosolic thymidine kinase. Purification and physical characterization of the enzyme from HeLa cells." in: The Journal of biological chemistry, Vol. 263, Issue 1, pp. 375-82, 1988 (PubMed).

Bradshaw, Deininger: "Human thymidine kinase gene: molecular cloning and nucleotide sequence of a cDNA expressible in mammalian cells." in: Molecular and cellular biology, Vol. 4, Issue 11, pp. 2316-20, 1985 (PubMed).

Welin, Kosinska, Mikkelsen et al.: "Structures of thymidine kinase 1 of human and mycoplasmic origin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 52, pp. 17970-5, 2004 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).