Did you know that you can buy products from over 140 different suppliers from us?

Thymidine Kinase 1, Soluble (TK1) antibody

Details for Product No. ABIN650533
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms TK2, Tk-1, Tk1a, Tk1b, D530002A18Rik, TK, Tk, fi19b06, zgc:55362, wu:fi19b06, TK1, tk2
Reactivity
Human
(52), (4), (3)
Host
Rabbit
(32), (20)
Clonality
Monoclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(45), (40), (10), (7), (6), (3), (2), (1)
Pubmed 4 references available
Quantity 100 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Catalog No. ABIN650533
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A synthetic peptide corresponding to residues in human Thymidine Kinase 1 was used as an immunogen.
Specificity A synthetic peptide corresponding to residues in human Thymidine Kinase 1 was used as an immunogen.
Alternative Name Thymidine Kinase 1
Background Cytosolic thymidine kinase 1, TK1, is a well known cell-cycle-regulated enzyme of importance in nucleotide metabolism as well as an activator of antiviral and anticancer drugs (1). TK1 catalyzes the ATP-dependent phosphorylation of thymidine to thymidine 5'-monophosphate, which is subsequently converted to thymidine 5'-triphosphate and utilized for DNA synthesis. In terminally differentiating cells, such as cardiac muscle cells, the cessation of DNA synthesis and withdrawal from the cell cycle are accompanied by the loss of TK1 activity (2). TK1 is a key enzyme of the salvage pathway for pyrimidine deoxyribonucleotide synthesis in a variety of organisms (3). Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle, it is very low in resting cells (4).
Molecular Weight 24 kDA
Gene ID 7083
UniProt P04183
Research Area Cell Signaling, Protein Modifications, Signaling, Metabolism, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-5000
Comment

Background: Cytosolic thymidine kinase 1, TK1, is a well known cell-cycle-regulated enzyme of importance in nucleotide metabolism as well as an activator of antiviral and anticancer drugs (1). TK1 catalyzes the ATP-dependent phosphorylation of thymidine to thymidine 5'-monophosphate, which is subsequently converted to thymidine 5'-triphosphate and utilized for DNA synthesis. In terminally differentiating cells, such as cardiac muscle cells, the cessation of DNA synthesis and withdrawal from the cell cycle are accompanied by the loss of TK1 activity (2). TK1 is a key enzyme of the salvage pathway for pyrimidine deoxyribonucleotide synthesis in a variety of organisms (3). Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle, it is very low in resting cells (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Thymidine Kinase 1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Sherley, Kelly: "Human cytosolic thymidine kinase. Purification and physical characterization of the enzyme from HeLa cells." in: The Journal of biological chemistry, Vol. 263, Issue 1, pp. 375-82, 1988 (PubMed).

Bradshaw, Deininger: "Human thymidine kinase gene: molecular cloning and nucleotide sequence of a cDNA expressible in mammalian cells." in: Molecular and cellular biology, Vol. 4, Issue 11, pp. 2316-20, 1985 (PubMed).

Welin, Kosinska, Mikkelsen et al.: "Structures of thymidine kinase 1 of human and mycoplasmic origin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 52, pp. 17970-5, 2004 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN650533
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
Did you look for something else?
back to top