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Toll-Like Receptor 2 (TLR2) (Extracellular) antibody

Details for Product No. ABIN650539
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Antigen
Synonyms TIL4, CD282, Ly105
Epitope
Extracellular
(20), (14), (6), (5), (5), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(274), (119), (17), (17), (13), (13), (12), (3), (1), (1), (1)
Host
Rabbit
(228), (73), (34), (5), (4), (3)
Clonality
Monoclonal
Conjugate
Un-conjugated
(41), (39), (26), (6), (5), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(194), (124), (93), (71), (42), (39), (30), (29), (24), (24), (20), (10), (3), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 100 µL
Options
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Catalog No. ABIN650539
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A synthetic peptide corresponding to residues surrounding human TLR2 was used as an immunogen.
Specificity A synthetic peptide corresponding to residues surrounding human TLR2 was used as an immunogen.
Alternative Name TLR-2
Background Toll-like receptor 2 (TLR2) is a signalling receptor that is activated by LPS in a response that depends on LPS-binding protein and is enhanced by CD14. A region in the intracellular domain of TLR2 with homology to a portion of the interleukin (IL)-1 receptor that is implicated in the activation of the IL-1-receptor-associated kinase is required for this response. Results indicate that TLR2 is a direct mediator of signalling by LPS (1). All bacterial pathogens produce lipoproteins (BLPs), which trigger the innate immune response. BLPs were found to induce apoptosis in THP-1 monocytic cells through human TLR2. BLPs also initiated apoptosis in an epithelial cell line transfected with TLR2. BLPs stimulated nuclear factor-kappaB, a transcriptional activator of multiple host defense genes, and activated the respiratory burst through TLR2. Thus, TLR2 is a molecular link between microbial products, apoptosis, and host defense mechanisms (2). It has been found that in TLR2 extracellular domain (C-term) all four predicted glycosylation sites are substituted, although one site is inefficiently core-glycosylated and its removal drastically affects secretion. The remaining TLR2 glycosylation sites also contribute to efficient protein secretion, albeit to a lesser degree (3).
Synonyms: Nos2, Nitric oxide synthase, inducible, NOS type II
Molecular Weight 90 kDA
Gene ID 7097
UniProt O60603
Research Area Cell Signaling, Protein Modifications, Cell Structure, Metabolism
Application Notes The suggested dilution is: WB: = 1:500-1000
Comment

Background: Toll-like receptor 2 (TLR2) is a signalling receptor that is activated by LPS in a response that depends on LPS-binding protein and is enhanced by CD14. A region in the intracellular domain of TLR2 with homology to a portion of the interleukin (IL)-1 receptor that is implicated in the activation of the IL-1-receptor-associated kinase is required for this response. Results indicate that TLR2 is a direct mediator of signalling by LPS (1). All bacterial pathogens produce lipoproteins (BLPs), which trigger the innate immune response. BLPs were found to induce apoptosis in THP-1 monocytic cells through human TLR2. BLPs also initiated apoptosis in an epithelial cell line transfected with TLR2. BLPs stimulated nuclear factor-kappaB, a transcriptional activator of multiple host defense genes, and activated the respiratory burst through TLR2. Thus, TLR2 is a molecular link between microbial products, apoptosis, and host defense mechanisms (2). It has been found that in TLR2 extracellular domain (C-term) all four predicted glycosylation sites are substituted, although one site is inefficiently core-glycosylated and its removal drastically affects secretion. The remaining TLR2 glycosylation sites also contribute to efficient protein secretion, albeit to a lesser degree (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment TLR-2 Antibody (extracellular) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Yang, Mark, Gray et al.: "Toll-like receptor-2 mediates lipopolysaccharide-induced cellular signalling." in: Nature, Vol. 395, Issue 6699, pp. 284-8, 1998 (PubMed).

Aliprantis, Yang, Mark et al.: "Cell activation and apoptosis by bacterial lipoproteins through toll-like receptor-2." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 736-9, 1999 (PubMed).

Weber, Morse, Gay: "Four N-linked glycosylation sites in human toll-like receptor 2 cooperate to direct efficient biosynthesis and secretion." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34589-94, 2004 (PubMed).

Hosts (228), (73), (34), (5), (4), (3)
Reactivities (274), (119), (17), (17), (13), (13), (12), (3), (1), (1), (1)
Applications (194), (124), (93), (71), (42), (39), (30), (29), (24), (24), (20), (10), (3), (1), (1), (1), (1)
Conjugates (41), (39), (26), (6), (5), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (20), (14), (6), (5), (5), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN650539
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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