Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3) antibody

Antigen: Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3)

Synonyms: A20, OTUD7C, TNFA1P2, MGC104522, MGC138687, MGC138688, Tnfip3, tnfaip3, MGC82339, TNFAIP3

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN650544

Additional Information

Alternative name: TNFAIP3

Gene ID: 7128

UniProt: P21580

Immunogen: A synthetic peptide corresponding to residues in human TNFAIP3 was used as an immunogen.

Description: Other names: Putative DNA-binding protein A20, Zinc finger protein A20

Characteristics: Rabbit Monoclonal

Specificity: A synthetic peptide corresponding to residues in human TNFAIP3 was used as an immunogen.

Molecular Weight: 80 kDA

Comments:

Background: Tumor necrosis factor, alpha-induced protein 3 (TNFAIP3) is a de-ubiquitinating enzyme that contains an N-terminal catalytic domain belonging to the ovarian-tumour superfamily of cysteine proteases (1). TNFAIP3 suppresses apoptosis. It is expressed in various cell types in response to a variety of stimuli that activate the transcription factor NF-kB, including Il-1, CD40 ligand, and PMA (3). TNFAIP3 has been strongly suggested as a key protein involved in tamoxifen resistance, and thus represents both a new breast cancer marker and a promising target for developing new strategies to prevent the emergence of acquired mechanisms of drug resistance in breast cancer (2).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 FC: ~~ 1:50 ICC: ~~ 1:100 IHC: ~~ 1:50~100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: TNFAIP3 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Apoptosis/Necrosis, Cell Signaling, Protein Modifications, Cell Structure, Signaling

Restrictions: For Research Use only

Publications

Product: Evans, Ovaa, Hamon et al.: "Zinc-finger protein A20, a regulator of inflammation and cell survival, has de-ubiquitinating activity." in: The Biochemical journal, Vol. 378, Issue Pt 3, pp. 727-34, 2004 (PubMed).

Daniel, Arvelo, Patel et al.: "A20 protects endothelial cells from TNF-, Fas-, and NK-mediated cell death by inhibiting caspase 8 activation." in: Blood, Vol. 104, Issue 8, pp. 2376-84, 2004 (PubMed).

Vendrell, Ghayad, Ben-Larbi et al.: "A20/TNFAIP3, a new estrogen-regulated gene that confers tamoxifen resistance in breast cancer cells." in: Oncogene, Vol. 26, Issue 32, pp. 4656-67, 2007 (PubMed).