Ubiquitin (C-Term) antibody

Details for Product No. ABIN650584
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
CG11624, DmUb, DmUbi-p63E, Dmel\\CG11624, Ub, Ubi-P63e, Ubi-p, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, ubi, ubi-p63E, ubi63E, CG18282, CG32744, CR32744, DmUbi-p5E, Dmel\\CG32744, ubiquitin, si:dz180g5.5, si: ... show more
CG11624, DmUb, DmUbi-p63E, Dmel\\CG11624, Ub, Ubi-P63e, Ubi-p, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, ubi, ubi-p63E, ubi63E, CG18282, CG32744, CR32744, DmUbi-p5E, Dmel\\CG32744, ubiquitin, si:dz180g5.5, si:busm1-180g5.5, LOC100194618, UBIQ, Ubiquitin, LOC100282068 show less
Epitope
C-Term
(13), (8), (7), (3), (3), (3), (2), (2), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(152), (109), (57), (56), (15), (15), (9), (8), (6), (6), (6), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host
Rabbit
(137), (117), (4), (2), (2), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(9), (6), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(186), (130), (84), (65), (38), (32), (24), (23), (21), (10), (2), (1)
Pubmed 3 references available
Quantity 100 μL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN650584
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A synthetic peptide corresponding to residues in the C-term of human Ubiquitin was used as immunogen.
Specificity A synthetic peptide corresponding to residues in the C-term of human Ubiquitin was used as immunogen.
Background Ubiquitin (originally ubiquitous imunopoeitic polypeptide-UBIP) is an 8.5 kDa protein highly conserved among eukaryotic species (1). Ubiquitin acts through its post-translational attachment (ubiquitinylation) to other proteins, where these modifications alter the function, location or trafficking of the protein and targets it for destruction by the 26S proteasome. Ubiquitinylation also termed ubiquination, is an ATP-dependent process that involves the action of at least three enzymes: E1 (activating enzyme), E2 (conjugation enzyme) and E3 (ligase) working sequentially (2-4). This process is an important regulatory tool that controls the concentration of key signaling and regulatory proteins as well as removing damaged, misfolded or mutant proteins (5).
Synonyms: UBC, UBCEP2, Ubiquitin
Molecular Weight 8.5 kDA
Gene ID 7311, 7314, 7316
UniProt P62988
Research Area Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000 ,IHC: = 1:100-250
Comment

Background: Ubiquitin (originally ubiquitous imunopoeitic polypeptide-UBIP) is an 8.5 kDa protein highly conserved among eukaryotic species (1). Ubiquitin acts through its post-translational attachment (ubiquitinylation) to other proteins, where these modifications alter the function, location or trafficking of the protein and targets it for destruction by the 26S proteasome. Ubiquitinylation also termed ubiquination, is an ATP-dependent process that involves the action of at least three enzymes: E1 (activating enzyme), E2 (conjugation enzyme) and E3 (ligase) working sequentially (2-4). This process is an important regulatory tool that controls the concentration of key signaling and regulatory proteins as well as removing damaged, misfolded or mutant proteins (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Ubiquitin Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Goldstein, Scheid, Hammerling et al.: "Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, Issue 1, pp. 11-5, 1975 (PubMed).

Gonen, Stancovski, Shkedy et al.: "Isolation, characterization, and partial purification of a novel ubiquitin-protein ligase, E3. Targeting of protein substrates via multiple and distinct recognition signals and conjugating enzymes." in: The Journal of biological chemistry, Vol. 271, Issue 1, pp. 302-10, 1996 (PubMed).

Hatakeyama, Nakayama: "U-box proteins as a new family of ubiquitin ligases." in: Biochemical and biophysical research communications, Vol. 302, Issue 4, pp. 635-45, 2003 (PubMed).

Validation Images
Did you look for something else?
back to top