Ubiquitin (C-Term) antibody

Antigen: Ubiquitin

Synonyms: MGC8385, FLJ25987, Ubc, Ubb2, Uba52, Rps27a, AL033289, CG11624, DmelCG11624, DmUb, Ub, ubi, Ubi-p, ubi-p63E, Ubi-P63e, ubi63E, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, UBC, CG18282, CG32744, CR32744, DmelCG32744, ubiquitin, si:dz180g5.5, si:busm1-180g5.5, UBB, Ubiquitin, CG11700, ESTS:143G11T, ESTS:199A6T, anon-sts41, poly-ub, DmelCR11700, LOC100194618, UBIQ, LOC100282068

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN650584

Additional Information

Gene ID: 7311, 7314, 7316, 6233

UniProt: P62988

Immunogen: A synthetic peptide corresponding to residues in the C-term of human Ubiquitin was used as immunogen.

Description: Other names: UBC, UBCEP2, Ubiquitin

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in the C-term of human Ubiquitin was used as immunogen.

Molecular Weight: 8.5 kDA

Comments:

Background: Ubiquitin (originally ubiquitous imunopoeitic polypeptide-UBIP) is an 8.5 kDa protein highly conserved among eukaryotic species (1). Ubiquitin acts through its post-translational attachment (ubiquitinylation) to other proteins, where these modifications alter the function, location or trafficking of the protein and targets it for destruction by the 26S proteasome. Ubiquitinylation also termed ubiquination, is an ATP-dependent process that involves the action of at least three enzymes: E1 (activating enzyme), E2 (conjugation enzyme) and E3 (ligase) working sequentially (2-4). This process is an important regulatory tool that controls the concentration of key signaling and regulatory proteins as well as removing damaged, misfolded or mutant proteins (5).

Synonyms: MGC8385, FLJ25987, Ubc, Ubb2, Uba52, Rps27a, AL033289, CG11624, DmelCG11624, DmUb, Ub, ubi, Ubi-p, ubi-p63E, Ubi-P63e, ubi63E, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, UBC, CG18282, CG32744, CR32744, DmelCG32744, ubiquitin, si:dz180g5.5, si:busm1-180g5.5, UBB, Ubiquitin, CG11700, ESTS:143G11T, ESTS:199A6T, anon-sts41, poly-ub, DmelCR11700, LOC100194618, UBIQ, LOC100282068

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000 IHC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Ubiquitin Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Goldstein, Scheid, Hammerling et al.: "Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, Issue 1, pp. 11-5, 1975 (PubMed).

Gonen, Stancovski, Shkedy et al.: "Isolation, characterization, and partial purification of a novel ubiquitin-protein ligase, E3. Targeting of protein substrates via multiple and distinct recognition signals and conjugating enzymes." in: The Journal of biological chemistry, Vol. 271, Issue 1, pp. 302-10, 1996 (PubMed).

Hatakeyama, Nakayama: "U-box proteins as a new family of ubiquitin ligases." in: Biochemical and biophysical research communications, Vol. 302, Issue 4, pp. 635-45, 2003 (PubMed).