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Ubiquitin (C-Term) antibody
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
|3 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Gene ID||7311, 7314, 7316, 6233|
|Immunogen||A synthetic peptide corresponding to residues in the C-term of human Ubiquitin was used as immunogen.|
|Description||Other names: UBC, UBCEP2, Ubiquitin|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues in the C-term of human Ubiquitin was used as immunogen.|
|Molecular Weight||8.5 kDA|
Background: Ubiquitin (originally ubiquitous imunopoeitic polypeptide-UBIP) is an 8.5 kDa protein highly conserved among eukaryotic species (1). Ubiquitin acts through its post-translational attachment (ubiquitinylation) to other proteins, where these modifications alter the function, location or trafficking of the protein and targets it for destruction by the 26S proteasome. Ubiquitinylation also termed ubiquination, is an ATP-dependent process that involves the action of at least three enzymes: E1 (activating enzyme), E2 (conjugation enzyme) and E3 (ligase) working sequentially (2-4). This process is an important regulatory tool that controls the concentration of key signaling and regulatory proteins as well as removing damaged, misfolded or mutant proteins (5).
|Synonyms||MGC8385, FLJ25987, Ubc, Ubb2, Uba52, Rps27a, AL033289, CG11624, DmelCG11624, DmUb, Ub, ubi, Ubi-p, ubi-p63E, Ubi-P63e, ubi63E, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, UBC, CG18282, CG32744, CR32744, DmelCG32744, ubiquitin, si:dz180g5.5, si:busm1-180g5.5, UBB, Ubiquitin, CG11700, ESTS:143G11T, ESTS:199A6T, anon-sts41, poly-ub, DmelCR11700, LOC100194618, UBIQ, LOC100282068|
|Application Notes||The suggested dilution is: WB: ~~ 1:1000 IHC: ~~ 1:250~500|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Ubiquitin Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
Goldstein, Scheid, Hammerling et al.: "Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, Issue 1, pp. 11-5, 1975 (PubMed).
Gonen, Stancovski, Shkedy et al.: "Isolation, characterization, and partial purification of a novel ubiquitin-protein ligase, E3. Targeting of protein substrates via multiple and distinct recognition signals and conjugating enzymes." in: The Journal of biological chemistry, Vol. 271, Issue 1, pp. 302-10, 1996 (PubMed).
Hatakeyama, Nakayama: "U-box proteins as a new family of ubiquitin ligases." in: Biochemical and biophysical research communications, Vol. 302, Issue 4, pp. 635-45, 2003 (PubMed).