Vesicle-Associated Membrane Protein 8 (Endobrevin) (VAMP8) (N-Term) antibody

Details for Product No. ABIN650585
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Antigen
Epitope
N-Term
(9), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(35), (19), (19)
Host
Rabbit
(31), (5), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(28), (10), (6), (4), (4), (3), (2), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 100 μL
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Immunogen A synthetic peptide corresponding to residues near the N-terminus of human VAMP8 was used as an immunogen.
Specificity A synthetic peptide corresponding to residues near the N-terminus of human VAMP8 was used as an immunogen.
Alternative Name VAMP8 (VAMP8 Antibody Abstract)
Background Synaptobrevins/vesicle-associated membrane proteins (VAMPs) together with syntaxins and a synaptosome-associated protein of 25 kDa (SNAP-25) are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane (1). The terminal step of cytokinesis in animal cells is the abscission of the midbody, a cytoplasmic bridge that connects the two prospective daughter cells. It has been shown that two members of the SNARE membrane fusion machinery, syntaxin 2 and endobrevin/VAMP8, specifically localize to the midbody during cytokinesis in mammalian cells (2). It has been established that VAMP8/endobrevin is a major player in regulated exocytosis of the exocrine pancreas. VAMP8 is enriched on the membrane of zymogen granules and exists in a complex with syntaxin 4 and SNAP-23. Results suggest a major physiological role of VAMP8 in regulated exocytosis of pancreatic acinar cells by serving as a v-SNARE of zymogen granules (3).
Synonyms: VAMP8, Vesicle-associated membrane protein 8, Endobrevin
Molecular Weight 17 kDA
Gene ID 8673
UniProt Q9BV40
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Structure
Application Notes IHC: = 1:100-250, WB: = 1:100000-1000000
Comment

Background: Synaptobrevins/vesicle-associated membrane proteins (VAMPs) together with syntaxins and a synaptosome-associated protein of 25 kDa (SNAP-25) are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane (1). The terminal step of cytokinesis in animal cells is the abscission of the midbody, a cytoplasmic bridge that connects the two prospective daughter cells. It has been shown that two members of the SNARE membrane fusion machinery, syntaxin 2 and endobrevin/VAMP8, specifically localize to the midbody during cytokinesis in mammalian cells (2). It has been established that VAMP8/endobrevin is a major player in regulated exocytosis of the exocrine pancreas. VAMP8 is enriched on the membrane of zymogen granules and exists in a complex with syntaxin 4 and SNAP-23. Results suggest a major physiological role of VAMP8 in regulated exocytosis of pancreatic acinar cells by serving as a v-SNARE of zymogen granules (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment VAMP8 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Wang, Ng, Lu et al.: "A role of VAMP8/endobrevin in regulated exocytosis of pancreatic acinar cells." in: Developmental cell, Vol. 7, Issue 3, pp. 359-71, 2004 (PubMed).

Low, Li, Miura et al.: "Syntaxin 2 and endobrevin are required for the terminal step of cytokinesis in mammalian cells." in: Developmental cell, Vol. 4, Issue 5, pp. 753-9, 2003 (PubMed).

Wong, Zhang, Xu et al.: "Endobrevin, a novel synaptobrevin/VAMP-like protein preferentially associated with the early endosome." in: Molecular biology of the cell, Vol. 9, Issue 6, pp. 1549-63, 1998 (PubMed).

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Catalog No. ABIN650585
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