Vimentin (VIM) (C-Term) antibody

Details for Product No. ABIN650593
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Antigen
Synonyms vime, CTRCT30, cb28, vim, vim1, vim2, VIM, Vimentin
Epitope
C-Term
(21), (19), (17), (16), (12), (8), (8), (7), (6), (6), (6), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(478), (165), (154), (85), (78), (52), (39), (17), (12), (12), (10), (8), (6), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(258), (229), (27), (9), (7), (5), (4)
Clonality
Monoclonal
Conjugate
Un-conjugated
(20), (19), (12), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
(400), (167), (166), (147), (104), (98), (64), (52), (40), (35), (10), (3), (3), (2), (2), (1), (1), (1)
Pubmed 4 references available
Quantity 100 μL
Options
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Catalog No. ABIN650593
450.00 $
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Immunogen A synthetic acetylated peptide corresponding to C-terminus of human Vimentin was used as an immunogen.
Specificity A synthetic acetylated peptide corresponding to C-terminus of human Vimentin was used as an immunogen.
Alternative Name Vimentin
Background Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is expressed during cell development and differentiation in variety of mescencymal cells and cell types derived from mesoderm (1). Vimentin is essential in the role of cell integrity and cytoskeletal stability. The reorganization of Vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). In particular, p21-activated kinase (PAK) phosphorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which induces Vimentin specific reorganization (3). During cytokinesis, Vimentin is regulated by Rho-kinase (ROCK) and Aurora B through phosphorylation at Ser38 and Ser72 (4). Also, coordinated by ROCK and Aurora B, Plk1 induces phosphorylation at Ser82 plays an important role in Vimentin segregation (5).
Synonyms: VIM, Vimentin
Molecular Weight 57 kDA
Gene ID 7341
UniProt P08670
Research Area Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-5000 ,IHC: = 1:250-500
Comment

Background: Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is expressed during cell development and differentiation in variety of mescencymal cells and cell types derived from mesoderm (1). Vimentin is essential in the role of cell integrity and cytoskeletal stability. The reorganization of Vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). In particular, p21-activated kinase (PAK) phosphorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which induces Vimentin specific reorganization (3). During cytokinesis, Vimentin is regulated by Rho-kinase (ROCK) and Aurora B through phosphorylation at Ser38 and Ser72 (4). Also, coordinated by ROCK and Aurora B, Plk1 induces phosphorylation at Ser82 plays an important role in Vimentin segregation (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Vimentin Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Eriksson, Opal, Goldman: "Intermediate filament dynamics." in: Current opinion in cell biology, Vol. 4, Issue 1, pp. 99-104, 1992 (PubMed).

Goto, Kosako, Tanabe et al.: "Phosphorylation of vimentin by Rho-associated kinase at a unique amino-terminal site that is specifically phosphorylated during cytokinesis." in: The Journal of biological chemistry, Vol. 273, Issue 19, pp. 11728-36, 1998 (PubMed).

Goto, Tanabe, Manser et al.: "Phosphorylation and reorganization of vimentin by p21-activated kinase (PAK)." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 7, Issue 2, pp. 91-7, 2002 (PubMed).

Yamaguchi, Goto, Yokoyama et al.: "Phosphorylation by Cdk1 induces Plk1-mediated vimentin phosphorylation during mitosis." in: The Journal of cell biology, Vol. 171, Issue 3, pp. 431-6, 2005 (PubMed).

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