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Vimentin (VIM) antibody

Antigen

Vimentin (VIM)
Synonyms FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)
4 references available
Certificates ISO 9001:2008
Catalog no. ABIN650593
Quantity 100ul  (Variants)
Price 450.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Vimentin
Gene ID 7341
UniProt P08670
Immunogen A synthetic acetylated peptide corresponding to C-terminus of human Vimentin was used as an immunogen.
Description Other names: VIM, Vimentin
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic acetylated peptide corresponding to C-terminus of human Vimentin was used as an immunogen.
Molecular Weight 57 kDA
Comments

Background: Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is expressed during cell development and differentiation in variety of mescencymal cells and cell types derived from mesoderm (1). Vimentin is essential in the role of cell integrity and cytoskeletal stability. The reorganization of Vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). In particular, p21-activated kinase (PAK) phosphorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which induces Vimentin specific reorganization (3). During cytokinesis, Vimentin is regulated by Rho-kinase (ROCK) and Aurora B through phosphorylation at Ser38 and Ser72 (4). Also, coordinated by ROCK and Aurora B, Plk1 induces phosphorylation at Ser82 plays an important role in Vimentin segregation (5).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:1000~5000 FC: ~~ 1:50 ICC: ~~ 1:100~250 IHC: ~~ 1:250~500
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Vimentin Antibody can be stored at -20°C for up to 12 months from time of receipt.
Research Area Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Eriksson, Opal, Goldman: "Intermediate filament dynamics." in: Current opinion in cell biology, Vol. 4, Issue 1, pp. 99-104, 1992 (PubMed).

Goto, Kosako, Tanabe et al.: "Phosphorylation of vimentin by Rho-associated kinase at a unique amino-terminal site that is specifically phosphorylated during cytokinesis." in: The Journal of biological chemistry, Vol. 273, Issue 19, pp. 11728-36, 1998 (PubMed).

Goto, Tanabe, Manser et al.: "Phosphorylation and reorganization of vimentin by p21-activated kinase (PAK)." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 7, Issue 2, pp. 91-7, 2002 (PubMed).

Yamaguchi, Goto, Yokoyama et al.: "Phosphorylation by Cdk1 induces Plk1-mediated vimentin phosphorylation during mitosis." in: The Journal of cell biology, Vol. 171, Issue 3, pp. 431-6, 2005 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Vimentin (VIM)", type "Antibodies"
Hosts Mouse (147), Rabbit (139), Chicken (17), Sheep (6), Guinea Pig (4), Rat (4), Goat (3)
Reactivities Human (264), Rat (Rattus) (112), Mouse (Murine) (103), Cow (Bovine) (63), Pig (Porcine) (57), Chicken (47), Dog (Canine) (38), Sheep (Ovine) (24), Horse (Equine) (18), Monkey (13), Goat (12), Mammalian (12), Hamster (10), Zebrafish (6), Guinea Pig (5), Amphibian (4), Xenopus laevis (3), Zebrafish (Danio rerio) (3), Potoroo (2), All Species (1), Cat (Feline) (1), Fish (1), Frog (1), Primate (1), Rabbit (1)
Applications Western Blotting (WB) (203), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (111), ELISA (109), Immunofluorescence (IF) (90), Immunocytochemistry (ICC) (76), Immunohistochemistry (IHC) (65), Flow Cytometry (FACS) (31), Immunohistochemistry (Frozen Sections) (IHC (fro)) (31), Immunoprecipitation (IP) (22), Immunohistochemistry (Fixed) (IHC (fx)) (15), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoelectrophoresis (IEP) (3), Intracellular Staining (IS) (3), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (ffpe)) (1), Transfected cell culture (TCC) (1)
Conjugates FITC (12), Biotin (11), Alexa Fluor 488 (7), PE (7), HRP (6), Dyomics 547 (4), Alexa Fluor 350 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), DY547 (2), DyLight 549 (2), Gold (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2)
Epitopes pSer82 (20), pSer72 (13), N-Term (11), Internal Region (6), pSer56 (6), C-Term (4), pSer38,N-Term (3), pSer72,Internal Region (3), pTyr61,N-Term (3), Ser82 (2), AA 2-466 (1), AA 300-350 (1), AA 400-500 (1), AA 70-120 (1), Center (1), pSer38 (1), pSer83 (1), pTyr996 (1)