|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Vimentin (VIM) antibody
|Synonyms||FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime|
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)
|4 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||A synthetic acetylated peptide corresponding to C-terminus of human Vimentin was used as an immunogen.|
|Description||Other names: VIM, Vimentin|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic acetylated peptide corresponding to C-terminus of human Vimentin was used as an immunogen.|
|Molecular Weight||57 kDA|
Background: Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is expressed during cell development and differentiation in variety of mescencymal cells and cell types derived from mesoderm (1). Vimentin is essential in the role of cell integrity and cytoskeletal stability. The reorganization of Vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). In particular, p21-activated kinase (PAK) phosphorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which induces Vimentin specific reorganization (3). During cytokinesis, Vimentin is regulated by Rho-kinase (ROCK) and Aurora B through phosphorylation at Ser38 and Ser72 (4). Also, coordinated by ROCK and Aurora B, Plk1 induces phosphorylation at Ser82 plays an important role in Vimentin segregation (5).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000~5000 FC: ~~ 1:50 ICC: ~~ 1:100~250 IHC: ~~ 1:250~500|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Vimentin Antibody can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
Eriksson, Opal, Goldman: "Intermediate filament dynamics." in: Current opinion in cell biology, Vol. 4, Issue 1, pp. 99-104, 1992 (PubMed).
Goto, Kosako, Tanabe et al.: "Phosphorylation of vimentin by Rho-associated kinase at a unique amino-terminal site that is specifically phosphorylated during cytokinesis." in: The Journal of biological chemistry, Vol. 273, Issue 19, pp. 11728-36, 1998 (PubMed).
Goto, Tanabe, Manser et al.: "Phosphorylation and reorganization of vimentin by p21-activated kinase (PAK)." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 7, Issue 2, pp. 91-7, 2002 (PubMed).
Yamaguchi, Goto, Yokoyama et al.: "Phosphorylation by Cdk1 induces Plk1-mediated vimentin phosphorylation during mitosis." in: The Journal of cell biology, Vol. 171, Issue 3, pp. 431-6, 2005 (PubMed).