|+1 404 474 4654|
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VLA alpha 4 antibody
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
|3 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||A synthetic peptide corresponding to residues near the C-terminus of human VLA-alpha 4 was used as an immunogen.|
|Description||Other names: ITGA4, CD49D, Integrin alpha-4, Integrin alpha-IV,VLA-4,CD49 antigen-like family member D|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues near the C-terminus of human VLA-alpha 4 was used as an immunogen.|
|Molecular Weight||150 kDA|
Background: VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, hematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000~2000 IP: ~~ 1:40 ICC: ~~ 1:50 IHC: ~~ 1:100~250|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||VLA alpha 4 Antibody can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Phospho-specific antibodies, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
Teixidó, Parker, Kassner et al.: "Functional and structural analysis of VLA-4 integrin alpha 4 subunit cleavage." in: The Journal of biological chemistry, Vol. 267, Issue 3, pp. 1786-91, 1992 (PubMed).
Takada, Elices, Crouse et al.: "The primary structure of the alpha 4 subunit of VLA-4: homology to other integrins and a possible cell-cell adhesion function." in: The EMBO journal, Vol. 8, Issue 5, pp. 1361-8, 1989 (PubMed).
Liu, Thomas, Woodside et al.: "Binding of paxillin to alpha4 integrins modifies integrin-dependent biological responses." in: Nature, Vol. 402, Issue 6762, pp. 676-81, 2000 (PubMed).