VLA alpha 4 antibody

Antigen: VLA alpha 4

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650602

Additional Information

Gene ID: 3676

UniProt: P13612

Immunogen: A synthetic peptide corresponding to residues near the C-terminus of human VLA-alpha 4 was used as an immunogen.

Description: Other names: ITGA4, CD49D, Integrin alpha-4, Integrin alpha-IV,VLA-4,CD49 antigen-like family member D

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the C-terminus of human VLA-alpha 4 was used as an immunogen.

Molecular Weight: 150 kDA

Comments:

Background: VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, hematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~2000 IP: ~~ 1:40 ICC: ~~ 1:50 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: VLA alpha 4 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Teixidó, Parker, Kassner et al.: "Functional and structural analysis of VLA-4 integrin alpha 4 subunit cleavage." in: The Journal of biological chemistry, Vol. 267, Issue 3, pp. 1786-91, 1992 (PubMed).

Takada, Elices, Crouse et al.: "The primary structure of the alpha 4 subunit of VLA-4: homology to other integrins and a possible cell-cell adhesion function." in: The EMBO journal, Vol. 8, Issue 5, pp. 1361-8, 1989 (PubMed).

Liu, Thomas, Woodside et al.: "Binding of paxillin to alpha4 integrins modifies integrin-dependent biological responses." in: Nature, Vol. 402, Issue 6762, pp. 676-81, 2000 (PubMed).