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Details for Product No. ABIN650603

Wiskott-Aldrich Syndrome-Like (WASL) antibody

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Antigen
Synonyms N-WASP, 2900021I12Rik, 3110031I02Rik, TRS4, IMD2, SCNX, THC, THC1, WASP, U42471, Wasp, NWASP, wasl, zgc:55478, zgc:77237, n-wasp, nwasp, WASL
Reactivity
»Alternatives Human, Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
Pubmed 3 references available
Catalog no. ABIN650603
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen A synthetic peptide corresponding to residues near the N-terminus of human WASP was used as an immunogen.
Specificity A synthetic peptide corresponding to residues near the N-terminus of human WASP was used as an immunogen.
Alternative Name WASP
Background The Wiskott-Aldrich syndrome (WAS) is an X-chromosome-linked recessive disease characterized by eczema, thrombocytopenia, and immunodeficiency (1). WAS is an immunodeficiency disorder with the most severe pathology in the T lymphocytes and platelets. The disease arises from mutations in the gene encoding the WAS protein (WASP). T lymphocytes of affected males with WAS exhibit a severe disturbance of the actin cytoskeleton, suggesting that the WASP could regulate its organization. Data suggest that the WASP might function as a signal transduction adaptor downstream of Cdc42, and in affected males, the cytoskeletal abnormalities may result from a defect in Cdc42 signaling (2). WASP is a key regulator of the Arp2/3 complex and the actin cytoskeleton in hematopoietic cells. WASP is capable of forming an auto-inhibited conformation, which can be disrupted by binding of Cdc42 and phosphatidylinositol 4,5-bisphosphate, leading to its activation. Stimulation of the collagen receptor on platelets and crosslinking the B-cell receptor induce tyrosine phosphorylation of WASP (3).
Synonyms: WAS, IMD2, Wiskott-Aldrich syndrome protein
Molecular Weight 60 kDA
Gene ID 7454
UniProt P42768
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:5000~10000 IP: ~~ 1:30 FC: ~~ 1:30 ICC: ~~ 1:100~250
Comment

Background: The Wiskott-Aldrich syndrome (WAS) is an X-chromosome-linked recessive disease characterized by eczema, thrombocytopenia, and immunodeficiency (1). WAS is an immunodeficiency disorder with the most severe pathology in the T lymphocytes and platelets. The disease arises from mutations in the gene encoding the WAS protein (WASP). T lymphocytes of affected males with WAS exhibit a severe disturbance of the actin cytoskeleton, suggesting that the WASP could regulate its organization. Data suggest that the WASP might function as a signal transduction adaptor downstream of Cdc42, and in affected males, the cytoskeletal abnormalities may result from a defect in Cdc42 signaling (2). WASP is a key regulator of the Arp2/3 complex and the actin cytoskeleton in hematopoietic cells. WASP is capable of forming an auto-inhibited conformation, which can be disrupted by binding of Cdc42 and phosphatidylinositol 4,5-bisphosphate, leading to its activation. Stimulation of the collagen receptor on platelets and crosslinking the B-cell receptor induce tyrosine phosphorylation of WASP (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment WASP Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Smith, Porter, Corden et al.: "Cloning of human, murine, and marsupial keratin 7 and a survey of K7 expression in the mouse." in: Biochemical and biophysical research communications, Vol. 297, Issue 4, pp. 818-27, 2002 (PubMed).

Coons, Estrada, Gamez et al.: "Cytokeratin CK 7 and CK 20 expression in pituitary adenomas." in: Endocrine pathology, Vol. 16, Issue 3, pp. 201-10, 2005 (PubMed).

Adley, Papavero, Sugimura et al.: "Diagnostic value of cytokeratin 7 and parvalbumin in differentiating chromophobe renal cell carcinoma from renal oncocytoma." in: Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology, Vol. 28, Issue 4, pp. 228-36, 2006 (PubMed).

Alternatives for antigen "Wiskott-Aldrich Syndrome-Like (WASL)", type "Antibodies"
Hosts (54), (4), (4)
Reactivities (61), (33), (27)
Applications (52), (26), (13), (10), (6), (6), (2), (2), (2)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (5), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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