anti-YAP1 antibody (Yes-Associated Protein 1) (C-Term)

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Antigen
Alternatives
Epitope
C-Term
(26), (24), (22), (14), (13), (13), (13), (12), (12), (10), (7), (6), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(195), (74), (70), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host
Rabbit
(153), (32), (9), (1)
Clonality
Monoclonal
Conjugate
This YAP1 antibody is un-conjugated
(7), (5), (5), (5), (5), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(148), (93), (49), (37), (29), (23), (16), (15), (11), (6), (4), (3), (1)
Pubmed 1 reference available
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Quantity 100 μL
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Immunogen A synthetic peptide corresponding to residues near the C-terminus of human YAP65 was used as an immunogen.
Specificity A synthetic peptide corresponding to residues near the C-terminus of human YAP65 was used as an immunogen.
Alternative Name YAP65 (YAP1 Antibody Abstract)
Background The transcriptional coactivator Yes-associated protein (YAP) has been shown to interact with and to enhance p73-dependent apoptosis in response to DNA damage. It has been shown that YAP requires the promyelocytic leukemia gene (PML) and nuclear body localization to coactivate p73. YAP imparts selectivity to p73 by promoting the activation of a subset of p53 and/or p73 target promoters (1). Akt promotes YAP localization to the cytoplasm, resulting in loss from the nucleus where it functions as a coactivator of transcription factors including p73. p73-mediated induction of Bax expression following DNA damage requires YAP function and is attenuated by Akt phosphorylation of YAP. YAP overexpression increases, while YAP depletion decreases, p73-mediated apoptosis following DNA damage, in an Akt inhibitable manner (2)
Synonyms: YAP1, YAP65, 65 kDa Yes-associated protein, YAP65
Molecular Weight 75 kDA
Gene ID 10413
UniProt P46937
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Pathways MAPK Signaling
Application Notes IHC: = 1:100-250, WB: = 1:100000
Comment

Background: The transcriptional coactivator Yes-associated protein (YAP) has been shown to interact with and to enhance p73-dependent apoptosis in response to DNA damage. It has been shown that YAP requires the promyelocytic leukemia gene (PML) and nuclear body localization to coactivate p73. YAP imparts selectivity to p73 by promoting the activation of a subset of p53 and/or p73 target promoters (1). Akt promotes YAP localization to the cytoplasm, resulting in loss from the nucleus where it functions as a coactivator of transcription factors including p73. p73-mediated induction of Bax expression following DNA damage requires YAP function and is attenuated by Akt phosphorylation of YAP. YAP overexpression increases, while YAP depletion decreases, p73-mediated apoptosis following DNA damage, in an Akt inhibitable manner (2)

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment YAP65 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Strano, Monti, Pediconi et al.: "The transcriptional coactivator Yes-associated protein drives p73 gene-target specificity in response to DNA Damage." in: Molecular cell, Vol. 18, Issue 4, pp. 447-59, 2005 (PubMed).