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Yes-Associated Protein 1 (YAP1) (pSer127) antibody

Details for Product No. ABIN650608
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Antigen
Synonyms YAP1, YAP, YAP2, YAP65, YKI, AI325207, Yap, Yap65, Yki, Yorkie, cb194, sb:cb194, si:ch211-181p1.5, si:dkey-3b8.3, wu:fc18c04, zgc:158380, xyap, yap, yap2, yap65, yki, HCCA1, HCCA2, YY1AP
Epitope
pSer127
(28), (14), (12), (12), (6), (5), (5), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(143), (70), (68), (37), (25), (24), (24), (3), (1), (1), (1)
Host
Rabbit
(118), (25), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(6), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(108), (61), (36), (32), (30), (23), (13), (9), (8), (6), (2), (1)
Pubmed 1 reference available
Quantity 100 µL
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Catalog No. ABIN650608
507.14 $
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Immunogen A phospho specific peptide corresponding to residues surrounding Ser127 of human YAP65 was used as immunogen. The antibody detects YAP65 phosphorylated at Serine 127.
Specificity A phospho specific peptide corresponding to residues surrounding Ser127 of human YAP65 was used as immunogen. The antibody detects YAP65 phosphorylated at Serine 127.
Alternative Name YAP65
Background The transcriptional coactivator Yes-associated protein (YAP, YAP65) has been shown to interact with and to enhance p73-dependent apoptosis in response to DNA damage. It has been shown that YAP requires the promyelocytic leukemia gene (PML) and nuclear body localization to coactivate p73. YAP imparts selectivity to p73 by promoting the activation of a subset of p53 and/or p73 target promoters (1). YAP binds with 14-3-3 after being phosphorylated by Akt at Ser127. Akt promotes YAP localization to the cytoplasm, resulting in loss from the nucleus where it functions as a coactivator of transcription factors including p73. p73-mediated induction of Bax expression following DNA damage requires YAP function and is attenuated by Akt phosphorylation of YAP. YAP overexpression increases, while YAP depletion decreases, p73-mediated apoptosis following DNA damage, in an Akt inhibitable manner (2).
Synonyms: YAP1, YAP65, 65 kDa Yes-associated protein, YAP65
Molecular Weight 65 - 75 kDA
Gene ID 10413
UniProt P46937
Research Area Ubiquitin-related antibodies, Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes IHC: = 1:100-250, WB: = 1:10000-50000
Comment

Background: The transcriptional coactivator Yes-associated protein (YAP, YAP65) has been shown to interact with and to enhance p73-dependent apoptosis in response to DNA damage. It has been shown that YAP requires the promyelocytic leukemia gene (PML) and nuclear body localization to coactivate p73. YAP imparts selectivity to p73 by promoting the activation of a subset of p53 and/or p73 target promoters (1). YAP binds with 14-3-3 after being phosphorylated by Akt at Ser127. Akt promotes YAP localization to the cytoplasm, resulting in loss from the nucleus where it functions as a coactivator of transcription factors including p73. p73-mediated induction of Bax expression following DNA damage requires YAP function and is attenuated by Akt phosphorylation of YAP. YAP overexpression increases, while YAP depletion decreases, p73-mediated apoptosis following DNA damage, in an Akt inhibitable manner (2).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment YAP65 Antibody Phospho (pS127) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Strano, Monti, Pediconi et al.: "The transcriptional coactivator Yes-associated protein drives p73 gene-target specificity in response to DNA Damage." in: Molecular cell, Vol. 18, Issue 4, pp. 447-59, 2005 (PubMed).

Validation Images
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