zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody

Antigen: zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)

Synonyms: SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN650610

Additional Information

Alternative name: ZAP-70

Gene ID: 7535

UniProt: P43403

Immunogen: A synthetic peptide corresponding to residues in N-terminus of human ZAP-70 was used as immunogen.

Description: Other names: ZAP70, SRK, Tyrosine-protein kinase ZAP-70, 70 kDa zeta-associated protein,Syk-related tyrosine kinase

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in N-terminus of human ZAP-70 was used as immunogen.

Molecular Weight: 70 kDA

Comments:

Background: Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500 IP: ~~ 1:30 FC: ~~ 1:40

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: ZAP-70 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Chromatin

Restrictions: For Research Use only

Publications

Product: Isakov, Wange, Burgess et al.: "ZAP-70 binding specificity to T cell receptor tyrosine-based activation motifs: the tandem SH2 domains of ZAP-70 bind distinct tyrosine-based activation motifs with varying affinity." in: The Journal of experimental medicine, Vol. 181, Issue 1, pp. 375-80, 1995 (PubMed).

Chu, Morita, Weiss: "The Syk family of protein tyrosine kinases in T-cell activation and development." in: Immunological reviews, Vol. 165, pp. 167-80, 1999 (PubMed).

Di Bartolo, Mège, Germain et al.: "Tyrosine 319, a newly identified phosphorylation site of ZAP-70, plays a critical role in T cell antigen receptor signaling." in: The Journal of biological chemistry, Vol. 274, Issue 10, pp. 6285-94, 1999 (PubMed).