zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (C-Term) antibody

Details for Product No. ABIN650611
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms zap70, MGC80670, MGC88962, ZAP70, 70kDa, AI327364, Srk, TZK, ZAP-70, mrtle, mur, SRK, STCD, STD
Epitope
C-Term
(40), (39), (25), (19), (19), (14), (11), (10), (7), (7), (7), (7), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(335), (160), (112), (49), (48), (37), (24), (24), (1)
Host
Rabbit
(239), (85), (8), (4), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(17), (14), (11), (9), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(209), (108), (95), (59), (50), (40), (27), (22), (17), (3), (2), (1)
Pubmed 3 references available
Quantity 100 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN650611
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A synthetic peptide corresponding to residues in C-terminus of human ZAP-70 was used as immunogen.
Specificity A synthetic peptide corresponding to residues in C-terminus of human ZAP-70 was used as immunogen.
Alternative Name ZAP-70
Background Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3).
Synonyms: ZAP70, SRK, Tyrosine-protein kinase ZAP-70, 70 kDa zeta-associated protein,Syk-related tyrosine kinase
Molecular Weight 70 kDA
Gene ID 7535
UniProt P43403
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Chromatin
Application Notes IHC: = 1:100-250, WB: = 1:1000-10000
Comment

Background: Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment ZAP-70 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Isakov, Wange, Burgess et al.: "ZAP-70 binding specificity to T cell receptor tyrosine-based activation motifs: the tandem SH2 domains of ZAP-70 bind distinct tyrosine-based activation motifs with varying affinity." in: The Journal of experimental medicine, Vol. 181, Issue 1, pp. 375-80, 1995 (PubMed).

Chu, Morita, Weiss: "The Syk family of protein tyrosine kinases in T-cell activation and development." in: Immunological reviews, Vol. 165, pp. 167-80, 1999 (PubMed).

Di Bartolo, Mège, Germain et al.: "Tyrosine 319, a newly identified phosphorylation site of ZAP-70, plays a critical role in T cell antigen receptor signaling." in: The Journal of biological chemistry, Vol. 274, Issue 10, pp. 6285-94, 1999 (PubMed).

Validation Images
Did you look for something else?
back to top