Finkel-Biskis-Reilly Murine Sarcoma Virus (FBR-MuSV) Ubiquitously Expressed (FAU) antibody

Antigen: Finkel-Biskis-Reilly Murine Sarcoma Virus (FBR-MuSV) Ubiquitously Expressed (FAU)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650648

Additional Information

Alternative name: FAU

Gene ID: 2197

UniProt: P35544

Immunogen: This FAU antibody is generated from rabbits immunized with human FAU recombinant protein.

Isotype: Ig

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This FAU antibody is generated from rabbits immunized with human FAU recombinant protein.

Molecular Weight: 14389 DA

Comments:

Background: FUBI is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It is a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Translation Factors, Protein Modifications, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of FAU Antibody (ABIN650648) in 293 cell line lysates (35ug/lane). FAU (arrow) was detected using the purified Pab.

FAU Antibody (ABIN650648) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Michiels, Van der Rauwelaert, Van Hasselt et al.: "fau cDNA encodes a ubiquitin-like-S30 fusion protein and is expressed as an antisense sequence in the Finkel-Biskis-Reilly murine sarcoma virus." in: Oncogene, Vol. 8, Issue 9, pp. 2537-46, 1993 (PubMed).

Vladimirov, Ivanov, Karpova et al.: "Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry." in: European journal of biochemistry / FEBS, Vol. 239, Issue 1, pp. 144-9, 1996 (PubMed).

Kenmochi, Kawaguchi, Rozen et al.: "A map of 75 human ribosomal protein genes." in: Genome research, Vol. 8, Issue 5, pp. 509-23, 1998 (PubMed).